Cited 7 times in
Experimental parathyroid hormone gene therapy using ØC31 integrase.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 홍순원 | - |
dc.contributor.author | 이유미 | - |
dc.contributor.author | 임승길 | - |
dc.contributor.author | 최한석 | - |
dc.date.accessioned | 2015-05-19T17:20:11Z | - |
dc.date.available | 2015-05-19T17:20:11Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0918-8959 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/107954 | - |
dc.description.abstract | ØC31 integrase can integrate targeted plasmid DNA into preferred locations in mammalian genomes, resulting in robust, long-term expression of the integrated transgene. This system represents an effective tool that opens up promising possibilities for gene therapy. The classical treatment for hypoparathyroidism was calcium and vitamin D replacement. Recently, parathyroid hormone (PTH) replacement was reported to be a more potentially physiologic treatment option. However, PTH synthesis is technically difficult and costly. These issues may be minimized by using PTH gene therapy. We attempted to achieve site-specific genomic integration of the PTH gene into a human cell line and mice using this system. We cotransfected 293 HEK cells with PTH-attB plasmid with or without ØC31 integrase plasmid. Expression and secretion of PTH into culture supernatants and site-specific genomic integration of PTH cDNA were assessed by immunoradiometric assays and pseudo-site analysis, respectively. In in vivo experiments, we injected the PTH-attB plasmid with or without ØC31 integrase plasmid into a mouse tail vein using the hydrodynamic method. Plasma PTH concentrations were serially measured, and site-specific integration of PTH cDNA into the mouse genome was confirmed by examining hepatic genomic DNA. PTH was expressed and secreted from 293 HEK cells and mouse hepatocytes, and pseudo-site analysis confirmed the site-specific integration of PTH cDNA into the host genomes. The site-specificity and efficiency of this system are advantageous in many areas, including, potentially, gene therapy. PTH gene therapy is one candidate; however, for clinical applications, we need to regulate PTH expression and secretion in the future. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1033~1041 | - |
dc.relation.isPartOf | ENDOCRINE JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Attachment Sites, Microbiological/genetics | - |
dc.subject.MESH | Bacteriophages/genetics | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Culture Media/chemistry | - |
dc.subject.MESH | Genetic Therapy/methods* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hypoparathyroidism/blood | - |
dc.subject.MESH | Hypoparathyroidism/genetics | - |
dc.subject.MESH | Hypoparathyroidism/pathology | - |
dc.subject.MESH | Hypoparathyroidism/therapy* | - |
dc.subject.MESH | Integrases/genetics* | - |
dc.subject.MESH | Integrases/physiology | - |
dc.subject.MESH | Liver/metabolism | - |
dc.subject.MESH | Liver/pathology | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred ICR | - |
dc.subject.MESH | Models, Theoretical | - |
dc.subject.MESH | Mutagenesis, Insertional/methods | - |
dc.subject.MESH | Parathyroid Hormone/analysis | - |
dc.subject.MESH | Parathyroid Hormone/blood | - |
dc.subject.MESH | Parathyroid Hormone/genetics* | - |
dc.subject.MESH | Parathyroid Hormone/metabolism | - |
dc.subject.MESH | Treatment Outcome | - |
dc.title | Experimental parathyroid hormone gene therapy using ØC31 integrase. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pathology (병리학) | - |
dc.contributor.googleauthor | Sihoon LEE | - |
dc.contributor.googleauthor | Soon Won HONG | - |
dc.contributor.googleauthor | Han Seok CHOI | - |
dc.contributor.googleauthor | Lee Young LEE | - |
dc.contributor.googleauthor | Chunja NAM | - |
dc.contributor.googleauthor | Yumie RHEE | - |
dc.contributor.googleauthor | Ung-il CHUNG | - |
dc.contributor.googleauthor | Sung-Kil LIM | - |
dc.identifier.doi | 10.1507/endocrj.K08E-040 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04411 | - |
dc.contributor.localId | A03012 | - |
dc.contributor.localId | A03375 | - |
dc.contributor.localId | A04206 | - |
dc.relation.journalcode | J00769 | - |
dc.identifier.eissn | 1348-4540 | - |
dc.identifier.pmid | 18689953 | - |
dc.subject.keyword | Gene therapy | - |
dc.subject.keyword | Parathyroid hormone | - |
dc.subject.keyword | Hypoparathyroidism | - |
dc.subject.keyword | ØC31 integrase | - |
dc.contributor.alternativeName | Hong, Soon Won | - |
dc.contributor.alternativeName | Rhee, Yumie | - |
dc.contributor.alternativeName | Lim, Sung Kil | - |
dc.contributor.alternativeName | Choi, Han Seok | - |
dc.contributor.affiliatedAuthor | Hong, Soon Won | - |
dc.contributor.affiliatedAuthor | Rhee, Yumie | - |
dc.contributor.affiliatedAuthor | Lim, Sung Kil | - |
dc.contributor.affiliatedAuthor | Choi, Han Seok | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 55 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1033 | - |
dc.citation.endPage | 1041 | - |
dc.identifier.bibliographicCitation | ENDOCRINE JOURNAL, Vol.55(6) : 1033-1041, 2008 | - |
dc.identifier.rimsid | 34804 | - |
dc.type.rims | ART | - |
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