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HIV 1형 RNA Viral Load 검출을 위한 두 가지 실시간 핵산증폭법의 비교
DC Field | Value | Language |
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dc.contributor.author | 김현숙 | - |
dc.date.accessioned | 2015-05-19T17:18:27Z | - |
dc.date.available | 2015-05-19T17:18:27Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1226-9336 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/107897 | - |
dc.description.abstract | Background: Detection of the viral load of Human Immunodeficiency Virus type 1 (HIV-1) RNA is important in clinical decision making and prognosis of HIV-infected patients. The aim of the study is to compare the performance of real-time RT-PCR (COBAS AmpliPrep/COBAS TaqMan HIV-1, CAP/CTM, Roche Diagnostics) and Nucleic Acid Sequence Based Amplification (NucliSens EasyQ HIV-1, NucliSens, BioMerieux) methods in Korean HIV-infected patients. Methods: Among the specimens requested for HIV-1 RNA viral load detection from 2005 to 2006, 153 specimens were selected based on the status of the specimens. CAP/CTM and NucliSens tests were performed according to the manufacturer’s instruction. Results: HIV-1 RNA is detected by both tests in 93 specimens. Among the remainder, CAP/CTM detected HIV-1 RNA in 10 specimens, while the same specimens showed results lower than the detection limit with NucliSens. Though results correlated appropriately (r=0.85, P<0.0001), mean differences between the two test results were ?0.1321 log10 IU/mL on Bland-Altman test. Conclusion: The methodologic difference or the presence of subtype may affect the agreement between the two tests. The standardization of methods and establishment of linear range in individual laboratory may be helpful to perform an accurate test | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 216~221 | - |
dc.relation.isPartOf | Korean Journal of Blood Transfusion (대한수혈학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | HIV 1형 RNA Viral Load 검출을 위한 두 가지 실시간 핵산증폭법의 비교 | - |
dc.title.alternative | Comparison of Two Commercial Real-time Nucleic Acid Amplification Methods for Detection of the Viral Load of Human Immunodeficiency Virus Type 1 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | 김수정 | - |
dc.contributor.googleauthor | 이은경 | - |
dc.contributor.googleauthor | 박윤희 | - |
dc.contributor.googleauthor | 김현숙 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01117 | - |
dc.relation.journalcode | J01970 | - |
dc.identifier.pmid | HIV ; Viral load ; RT-PCR ; NASBA | - |
dc.subject.keyword | HIV | - |
dc.subject.keyword | Viral load | - |
dc.subject.keyword | RT-PCR | - |
dc.subject.keyword | NASBA | - |
dc.contributor.alternativeName | Kim, Hyon Suk | - |
dc.contributor.affiliatedAuthor | Kim, Hyon Suk | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 19 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 216 | - |
dc.citation.endPage | 221 | - |
dc.identifier.bibliographicCitation | Korean Journal of Blood Transfusion (대한수혈학회지), Vol.19(3) : 216-221, 2008 | - |
dc.identifier.rimsid | 34761 | - |
dc.type.rims | ART | - |
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