Cited 5 times in
Identification of the repeated number of C and D regions of tyrosine phosphorylation motifs in Helicobacter pylori cagA using multiplex PCR.
DC Field | Value | Language |
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dc.contributor.author | 문병수 | - |
dc.date.accessioned | 2015-05-19T17:12:12Z | - |
dc.date.available | 2015-05-19T17:12:12Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0385-5600 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/107698 | - |
dc.description.abstract | Various tyrosine phosphorylation motif regions of H. pylori cagA exist. The number of these regions was found to have some influence on cell signaling, which was found to be more pronounced when in D (ESS) region than in C (WSS) region. A molecular biological method with multiplex PCR was developed to distinguish C and D regions, and to identify the repetition number of tyrosine phosphorylation of the cagA gene. Multiplex PCR using novel primer sets was performed on 73 strains of H. pylori isolated from Korean patients with upper gastrointestinal diseases. The Western cagA was identified in only 3 strains (4.1%) whereas East Asia cagA was identified in 69 strains (94.5%). These results were reconfirmed through a sequencing analysis. The method developed in this study would be useful for monitoring the repeated number of C and D regions of tyrosine phosphorylation motifs in H. pylori cagA | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 479~483 | - |
dc.relation.isPartOf | MICROBIOLOGY AND IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amino Acid Motifs | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Antigens, Bacterial/genetics* | - |
dc.subject.MESH | Antigens, Bacterial/metabolism | - |
dc.subject.MESH | Bacterial Proteins/genetics* | - |
dc.subject.MESH | Bacterial Proteins/metabolism | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | DNA, Bacterial/chemistry | - |
dc.subject.MESH | DNA, Bacterial/genetics | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Helicobacter Infections/immunology* | - |
dc.subject.MESH | Helicobacter pylori/genetics* | - |
dc.subject.MESH | Helicobacter pylori/metabolism | - |
dc.subject.MESH | Helicobacter pylori/pathogenicity | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | Sequence Alignment | - |
dc.subject.MESH | Stomach Diseases/microbiology* | - |
dc.title | Identification of the repeated number of C and D regions of tyrosine phosphorylation motifs in Helicobacter pylori cagA using multiplex PCR. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Byungrak An | - |
dc.contributor.googleauthor | Gyusang Lee | - |
dc.contributor.googleauthor | Kwanhun Lim | - |
dc.contributor.googleauthor | Byung Soo Moon | - |
dc.contributor.googleauthor | Jong Bae Kim | - |
dc.identifier.doi | 10.1111/j.1348-0421.2008.00064.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01356 | - |
dc.relation.journalcode | J02232 | - |
dc.identifier.eissn | 1348-0421 | - |
dc.identifier.pmid | 18822081 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1348-0421.2008.00064.x/abstract | - |
dc.subject.keyword | ESS | - |
dc.subject.keyword | Helicobacter pylori cagA | - |
dc.subject.keyword | multiplex PCR | - |
dc.subject.keyword | WSS | - |
dc.contributor.alternativeName | Moon, Byung Soo | - |
dc.contributor.affiliatedAuthor | Moon, Byung Soo | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 52 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 479 | - |
dc.citation.endPage | 483 | - |
dc.identifier.bibliographicCitation | MICROBIOLOGY AND IMMUNOLOGY, Vol.52(10) : 479-483, 2008 | - |
dc.identifier.rimsid | 54848 | - |
dc.type.rims | ART | - |
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