Cited 85 times in
Efficient derivation of functional dopaminergic neurons from human embryonic stem cells on a large scale.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김동욱 | - |
dc.date.accessioned | 2015-05-19T17:11:39Z | - |
dc.date.available | 2015-05-19T17:11:39Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1754-2189 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/107680 | - |
dc.description.abstract | Cell-replacement therapy using human embryonic stem cells (hESCs) holds great promise in treating Parkinson's disease. We have recently reported a highly efficient method to generate functional dopaminergic (DA) neurons from hESCs. Our method includes a unique step, the formation of spherical neural masses (SNMs), and offers the highest yield of DA neurons ever achieved so far. In this report, we describe our method step by step, covering not only how to differentiate hESCs into DA neurons at a high yield, but also how to amplify, freeze and thaw the SNMs, which are the key structures that make our protocol unique and advantageous. Although the whole process of generation of DA neurons from hESCs takes about 2 months, only 14 d are needed to derive DA neurons from the SNMs. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1888~1894 | - |
dc.relation.isPartOf | NATURE PROTOCOLS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Culture Techniques* | - |
dc.subject.MESH | Cell Differentiation* | - |
dc.subject.MESH | Dopamine/metabolism* | - |
dc.subject.MESH | Embryonic Stem Cells/cytology* | - |
dc.subject.MESH | Embryonic Stem Cells/physiology | - |
dc.subject.MESH | Embryonic Stem Cells/transplantation | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Neurons/cytology* | - |
dc.subject.MESH | Neurons/metabolism | - |
dc.subject.MESH | Receptors, Dopamine/metabolism | - |
dc.title | Efficient derivation of functional dopaminergic neurons from human embryonic stem cells on a large scale. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학) | - |
dc.contributor.googleauthor | Myung-Soo Cho | - |
dc.contributor.googleauthor | Dong-Youn Hwang | - |
dc.contributor.googleauthor | Dong-Wook Kim | - |
dc.identifier.doi | 10.1038/nprot.2008.188 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00406 | - |
dc.relation.journalcode | J02300 | - |
dc.identifier.eissn | 1750-2799 | - |
dc.identifier.pmid | 19008875 | - |
dc.identifier.url | http://www.nature.com/nprot/journal/v3/n12/full/nprot.2008.188.html | - |
dc.subject.keyword | behavioral recovery | - |
dc.subject.keyword | differentiation | - |
dc.subject.keyword | Parkinson disease | - |
dc.subject.keyword | cell therapy | - |
dc.subject.keyword | spherical neural mass | - |
dc.contributor.alternativeName | Kim, Dong Wook | - |
dc.contributor.affiliatedAuthor | Kim, Dong Wook | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 3 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1888 | - |
dc.citation.endPage | 1894 | - |
dc.identifier.bibliographicCitation | NATURE PROTOCOLS, Vol.3(12) : 1888-1894, 2008 | - |
dc.identifier.rimsid | 54834 | - |
dc.type.rims | ART | - |
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