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Trichomonas vaginalis: reactive oxygen species mediates caspase-3 dependent apoptosis of human neutrophils

Authors
 Hyun-Ouk Song  ;  Myeong-Heon Shin  ;  Myoung-Hee Ahn  ;  Duk-Young Min  ;  Yong-Seok Kim  ;  Jae-Sook Ryu 
Citation
 EXPERIMENTAL PARASITOLOGY, Vol.118(1) : 59-65, 2008 
Journal Title
 EXPERIMENTAL PARASITOLOGY 
ISSN
 0014-4894 
Issue Date
2008
MeSH
Animals ; Apoptosis/physiology* ; Caspase 3/metabolism* ; Enzyme Inhibitors/pharmacology ; Humans ; Membrane Potential, Mitochondrial/drug effects ; NADPH Oxidases/antagonists & inhibitors ; NADPH Oxidases/metabolism ; Neutrophils/cytology* ; Neutrophils/drug effects ; Neutrophils/metabolism ; Onium Compounds/pharmacology ; Reactive Oxygen Species/metabolism* ; Receptors, IgG/metabolism ; Trichomonas vaginalis/metabolism*
Keywords
Animals ; Apoptosis/physiology* ; Caspase 3/metabolism* ; Enzyme Inhibitors/pharmacology ; Humans ; Membrane Potential, Mitochondrial/drug effects ; NADPH Oxidases/antagonists & inhibitors ; NADPH Oxidases/metabolism ; Neutrophils/cytology* ; Neutrophils/drug effects ; Neutrophils/metabolism ; Onium Compounds/pharmacology ; Reactive Oxygen Species/metabolism* ; Receptors, IgG/metabolism ; Trichomonas vaginalis/metabolism*
Abstract
There are many neutrophils in the vaginal discharge from women infected with Trichomonas vaginalis. The aim of our study was to determine whether human neutrophil apoptosis may be regulated by reactive oxygen species (ROS) in response to trichomonads infection. Incubation of human neutrophils with live trichomonads caused marked receptor shedding of CD16, decrease of mitochondrial membrane potential (MMP) and caspase-3 activation in human neutrophils. These proapoptotic effects of T. vaginalis on neutrophils were inhibited by pretreatment of neutrophils with an inhibitor of NADPH oxidase, diphenyleneiodonium chloride (DPI), suggesting an important role of intracellular ROS accumulation in T. vaginalis-triggered apoptosis. Indeed, large amounts of ROS levels were detected in neutrophils incubated with live trichomonads, and were also effectively inhibited by DPI. However, pan-caspase inhibitor z-VAD-fmk or caspase-3 inhibitor z-DEVD-fmk did not affect T. vaginalis-induced ROS generation in neutrophils. These results suggest that ROS-dependent caspase-3 activation plays an important role in apoptosis of human neutrophils induced by T. vaginalis
Full Text
http://www.sciencedirect.com/science/article/pii/S0014489407001749
DOI
10.1016/j.exppara.2007.06.010
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107669
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