혈관이완제의 전처치가 토끼의 상완동맥과 복강동맥의 혈관수축에 미치는 효과 - Nitroglycerin, Nicardipine, Verapamil과 Papaverine의 비교

Abstract

Background: Vasoconstrictor-induced reduction in arterial graft diameter can cause significant flow deprivation. The aim of this study was to evaluate the effect of vasodilator pretreatment on vasoconstrictor-induced blood vessel spasm in vitro. Material and Method: Rabbit brachial arteries (BA) and celiac arteries (CA) were cut into rings (3 ∼4 mm) and suspended with a force displacement transducer (TSD 125CⓇ, Biopac Inc. USA) in a tissue bath fil- led with 5 mL modified Krebs solution bubbled with 5% CO2 and 95% O2 at 38 o C. The rings were contracted with vasoconstrictors, and the developed tension changes were considered control values. The rings were then pre- treated with 30μM nitroglycerin, nicardipine, verapamil, and papaverine, respectively, for 40 minutes and rinsed with the physiologic buffered salt solution three times every 15 min. The vasoconstrictor-induced tension changes after the previous procedure were considered experimental values. Data are expressed as the percentage tension induced by vasoconstrictors before and after pretreatment with vasodilators. Result: Nicardipine depressed vaso- constriction induced by norepinephrine, angiotensin II (AII), and U46619 in both the BA and the CA more sig- nificantly than did nitroglycerin (p＜0.01) and verapamil (p＜0.05). Verapamil depressed vasoconstriction induced by 5-hydroxytryptamine (5HT), AII, and U46619 in the BA and by 5HT in the CA more significantly than did nitro- glycerin (p＜0.01). Conclusion: These findings suggest that both nicardipine and verapamil effectively depressed vasoconstrictor action. Nicardipine is thought to be more effective than verapamil for the prevention of vaso- constrictor action