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Molecular mechanisms of cell death of mycophenolic acid-treated primary isolated rat islets: implication of mitogen-activated protein kinase activation

DC Field Value Language
dc.contributor.author강창무-
dc.contributor.author김유선-
dc.contributor.author허규하-
dc.date.accessioned2015-05-19T17:08:52Z-
dc.date.available2015-05-19T17:08:52Z-
dc.date.issued2008-
dc.identifier.issn0041-1345-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/107591-
dc.description.abstractOptimal immunosuppression after pancreas islet transplantation has not yet been established to achieve long-term graft survival. Mycophenolic acid (MPA) is widely used as an immunosuppressive drug after transplantation including among recipients of pancreas islet cells. Previously, we reported MPA-induced islet apoptosis in the HIT-T15 cell line. In this study, we confirmed the effects of MPA on cell death and its potential implications on the mitogen-activated protein kinase (MAPK) family expression levels in primary isolated rat islets. Lewis islets isolated by collagenase digestion were purified by the density gradient method. Cell death was analyzed by methylthiazoletetrazolium assay. Activation of MAPK kinase 4 (MKK4), c-jun N-terminal protein kinase (JNK), p38 MAPK, and caspase-3 cleavage was examined by Western blot analyses. MPA treatments (> 25 micromol/L) increased cell death significantly at 24 hours and in a dose-dependent manner activated MKK4, JNK, and p38 MAPK at 20 hours. Caspase-3 cleavage was also increased by MPA treatment. These results suggested that MPA induced significant cell death among primary isolated rat islets by activation of MKK4, JNK, and p38 MAPK, as well as caspase-3 cleavage.-
dc.description.statementOfResponsibilityopen-
dc.format.extent2575~2577-
dc.relation.isPartOfTRANSPLANTATION PROCEEDINGS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCaspase 3/metabolism-
dc.subject.MESHCell Death/drug effects*-
dc.subject.MESHEnzyme Activation-
dc.subject.MESHIslets of Langerhans/cytology*-
dc.subject.MESHIslets of Langerhans/drug effects*-
dc.subject.MESHIslets of Langerhans/enzymology-
dc.subject.MESHJNK Mitogen-Activated Protein Kinases/metabolism-
dc.subject.MESHKinetics-
dc.subject.MESHMitogen-Activated Protein Kinases/metabolism*-
dc.subject.MESHMycophenolic Acid/pharmacology*-
dc.subject.MESHRats-
dc.subject.MESHRats, Inbred Lew-
dc.subject.MESHp38 Mitogen-Activated Protein Kinases/metabolism-
dc.titleMolecular mechanisms of cell death of mycophenolic acid-treated primary isolated rat islets: implication of mitogen-activated protein kinase activation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Surgery (외과학)-
dc.contributor.googleauthorJ.Y. Kim-
dc.contributor.googleauthorK.H. Huh-
dc.contributor.googleauthorY.-J. Park-
dc.contributor.googleauthorY. Fang-
dc.contributor.googleauthorC.M. Kang-
dc.contributor.googleauthorY.S. Kim-
dc.identifier.doi10.1016/j.transproceed.2008.07.123-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00088-
dc.contributor.localIdA00785-
dc.contributor.localIdA04344-
dc.relation.journalcodeJ02755-
dc.identifier.eissn1873-2623-
dc.identifier.pmid18929805-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0041134508009779-
dc.subject.keywordAnimals-
dc.subject.keywordCaspase 3/metabolism-
dc.subject.keywordCell Death/drug effects*-
dc.subject.keywordEnzyme Activation-
dc.subject.keywordIslets of Langerhans/cytology*-
dc.subject.keywordIslets of Langerhans/drug effects*-
dc.subject.keywordIslets of Langerhans/enzymology-
dc.subject.keywordJNK Mitogen-Activated Protein Kinases/metabolism-
dc.subject.keywordKinetics-
dc.subject.keywordMitogen-Activated Protein Kinases/metabolism*-
dc.subject.keywordMycophenolic Acid/pharmacology*-
dc.subject.keywordRats-
dc.subject.keywordRats, Inbred Lew-
dc.subject.keywordp38 Mitogen-Activated Protein Kinases/metabolism-
dc.contributor.alternativeNameKang, Chang Moo-
dc.contributor.alternativeNameKim, Yu Seun-
dc.contributor.alternativeNameHuh, Kyu Ha-
dc.contributor.affiliatedAuthorKang, Chang Moo-
dc.contributor.affiliatedAuthorKim, Yu Seun-
dc.contributor.affiliatedAuthorHuh, Kyu Ha-
dc.rights.accessRightsnot free-
dc.citation.volume40-
dc.citation.number8-
dc.citation.startPage2575-
dc.citation.endPage2577-
dc.identifier.bibliographicCitationTRANSPLANTATION PROCEEDINGS, Vol.40(8) : 2575-2577, 2008-
dc.identifier.rimsid53327-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

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