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Application of a peptide-based PF2D platform for quantitative proteomics in disease biomarker discovery

Authors
 Hyoung-Joo Lee  ;  Min-Jeong Kang  ;  Eun-Young Lee  ;  Sang Yun Cho  ;  Hoguen Kim  ;  Young-Ki Paik 
Citation
 PROTEOMICS, Vol.8(16) : 3371-3381, 2008 
Journal Title
PROTEOMICS
ISSN
 1615-9853 
Issue Date
2008
MeSH
Adult ; Alkaline Phosphatase/metabolism ; Biomarkers/analysis* ; Biomarkers/blood ; Carcinoma, Hepatocellular/blood ; Carcinoma, Hepatocellular/diagnosis* ; Carcinoma, Hepatocellular/metabolism ; Chemical Fractionation/methods* ; Chromatography, Liquid ; Electrophoresis, Gel, Two-Dimensional ; Humans ; Male ; Peptides/chemistry ; Peptides/metabolism* ; Proteomics/methods* ; Reproducibility of Results ; Tandem Mass Spectrometry
Keywords
Biomarker ; HCC ; Peptide ; PF2D ; Quantitative proteomics
Abstract
A peptide-based 2-D liquid phase fractionation (PF2D) system was used in a quantitative proteomic analysis of hepatocellular carcinoma. 2-D liquid maps of peptide specimens showed better resolution than those of proteins, leading to the identification of differentially expressed proteins. Peptide-based PF2D gave well-matched theoretical and experimental pI values and was proven to be a very efficient and versatile analytical tool for both large-scale profiling and quantification of phosphoproteins in disease biomarker discovery.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/pmic.200800111/abstract
DOI
10.1002/pmic.200800111
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hogeun(김호근)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107561
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