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Calmodulin dynamically regulates the trafficking of the metabotropic glutamate receptor mGluR5

Authors
 Jeong Ho Lee  ;  Jinu Lee  ;  Kyu Yeong Choi  ;  Regine Hepp  ;  Jae-Youn Lee  ;  Mi Kyung Lim  ;  Mayumi Chatani-Hinze  ;  Paul A. Roche  ;  Dong Goo Kim  ;  Young Soo Ahn  ;  Chul Hoon Kim  ;  Katherine W. Roche 
Citation
 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol.105(34) : 12575-12580, 2008 
Journal Title
 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 
ISSN
 0027-8424 
Issue Date
2008
MeSH
Animals ; Calcium Signaling ; Calmodulin/physiology* ; Cells, Cultured ; Hippocampus/cytology ; Phosphorylation ; Protein Binding ; Protein Kinase C/metabolism ; Protein Transport ; Rats ; Rats, Sprague-Dawley ; Receptor, Metabotropic Glutamate 5 ; Receptors, Metabotropic Glutamate/metabolism* ; Signal Transduction ; Transfection
Keywords
phosphorylation ; protein kinase C ; receptor trafficking
Abstract
Metabotropic glutamate receptors (mGluRs) 1-8 are G protein-coupled receptors (GPCRs) that modulate excitatory neurotransmission, neurotransmitter release, and synaptic plasticity. PKC regulates many aspects of mGluR function, including protein-protein interactions, Ca(2+) signaling, and receptor desensitization. However, the mechanisms by which PKC regulates mGluR function are poorly understood. We have now identified calmodulin (CaM) as a dynamic regulator of mGluR5 trafficking. We show that the major PKC phosphorylation site on the intracellular C terminus of mGluR5 is serine 901 (S901), and phosphorylation of this residue is up-regulated in response to both receptor and PKC activation. In addition, S901 phosphorylation inhibits mGluR5 binding to CaM, decreasing mGluR5 surface expression. Furthermore, blocking PKC phosphorylation of mGluR5 on S901 dramatically affects mGluR5 signaling by prolonging Ca(2+) oscillations. Thus, our data demonstrate that mGluR5 activation triggers phosphorylation of S901, thereby directly linking PKC phosphorylation, CaM binding, receptor trafficking, and downstream signaling
Files in This Item:
T200801113.pdf Download
DOI
10.1073/pnas.0712033105
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Goo(김동구)
Kim, Chul Hoon(김철훈) ORCID logo https://orcid.org/0000-0002-7360-429X
Ahn, Young Soo(안영수)
Lee, Jae Youn(이재연)
Lee, Jeong Ho(이정호)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107389
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