Cited 8 times in
Quantitative determination of progesterones and corticosteroids in human urine using gas chromatography/mass spectrometry: application to pelvic organ prolapse patients
DC Field | Value | Language |
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dc.contributor.author | 배상욱 | - |
dc.date.accessioned | 2015-05-19T17:00:43Z | - |
dc.date.available | 2015-05-19T17:00:43Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0951-4198 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/107360 | - |
dc.description.abstract | A quantitative analytical method using gas chromatography/mass spectrometry (GC/MS) to determine urinary concentrations of eight progesterones and corticosteroids has been developed. After enzymatic hydrolysis with beta-glucouronidase/arylsulfatase, urine samples were extracted by simple one-step solid-phase extraction. Obtained extracts were derivatized with a mixture of N-methyl-N-(trimethylsilyl)trifluoroacetamide/ammonium iodide/dithiothreitol and determined by GC/MS in selected ion monitoring mode to increase the sensitivity. d(4)-Cortisol and d(9)-progesterone were used as internal standards for two different steroid groups. The linear correlation coefficient was in the range of 0.9913 to 0.9998 and recoveries were over 80% for all compounds. Precision and accuracy were in the range of 0.9-18.1 and 84.1-118.7%, respectively. The limit of quantitation (LOQ) was 10 ng/mL for 11-deoxycorticosterone and 21-deoxycortisol and 5 ng/mL for all other analytes. The developed method was successfully applied on pelvic organ prolapsed patients (n = 10, age: 67.9 +/- 4.9) and post-menopausal (n = 10, age: 63.6 +/- 5.5) control women. Urinary levels of most progesterones and corticosteroids except 11-deoxycorticosterone decreased but only that of 17 alpha-hydroxyprogesterone significantly decreased in patients compared with the control groups. Thus, it is concluded that progesterones could be a factor in the pathogenesis of pelvic organ prolapse, and, among them, 17 alpha-hydroxyprogesterone could be a biomarker for pelvic organ prolapse. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 959~964 | - |
dc.relation.isPartOf | RAPID COMMUNICATIONS IN MASS SPECTROMETRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adrenal Cortex Hormones/urine* | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Biomarkers/urine | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gas Chromatography-Mass Spectrometry/methods* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Pelvic Floor | - |
dc.subject.MESH | Postmenopause/urine* | - |
dc.subject.MESH | Progesterone/urine* | - |
dc.subject.MESH | Reproducibility of Results | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | Urinalysis/methods* | - |
dc.subject.MESH | Uterine Prolapse/diagnosis | - |
dc.subject.MESH | Uterine Prolapse/urine* | - |
dc.title | Quantitative determination of progesterones and corticosteroids in human urine using gas chromatography/mass spectrometry: application to pelvic organ prolapse patients | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Obstetrics & Gynecology (산부인과학) | - |
dc.contributor.googleauthor | Naz Shama | - |
dc.contributor.googleauthor | Sang Wook Bai | - |
dc.contributor.googleauthor | Bong Chul Chung | - |
dc.contributor.googleauthor | Byung Hwa Jung | - |
dc.identifier.doi | 10.1002/rcm.3441 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01793 | - |
dc.relation.journalcode | J03216 | - |
dc.identifier.eissn | 1097-0231 | - |
dc.identifier.pmid | 18320550 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/rcm.3441/abstract | - |
dc.subject.keyword | Adrenal Cortex Hormones/urine* | - |
dc.subject.keyword | Aged | - |
dc.subject.keyword | Biomarkers/urine | - |
dc.subject.keyword | Female | - |
dc.subject.keyword | Gas Chromatography-Mass Spectrometry/methods* | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Pelvic Floor | - |
dc.subject.keyword | Postmenopause/urine* | - |
dc.subject.keyword | Progesterone/urine* | - |
dc.subject.keyword | Reproducibility of Results | - |
dc.subject.keyword | Sensitivity and Specificity | - |
dc.subject.keyword | Urinalysis/methods* | - |
dc.subject.keyword | Uterine Prolapse/diagnosis | - |
dc.subject.keyword | Uterine Prolapse/urine* | - |
dc.contributor.alternativeName | Bai, Sang Wook | - |
dc.contributor.affiliatedAuthor | Bai, Sang Wook | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 22 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 959 | - |
dc.citation.endPage | 964 | - |
dc.identifier.bibliographicCitation | RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Vol.22(7) : 959-964, 2008 | - |
dc.identifier.rimsid | 45066 | - |
dc.type.rims | ART | - |
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