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A 27 kDa Cysteine Protease Secreted by Newly Excysted Paragonimus westermani Metacercariae Induces Superoxide Anion Production and Degranulation of Human Eosinophils

DC FieldValueLanguage
dc.contributor.author신명헌-
dc.date.accessioned2015-05-19T16:57:06Z-
dc.date.available2015-05-19T16:57:06Z-
dc.date.issued2008-
dc.identifier.issn0023-4001-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/107247-
dc.description.abstractEosinophil degranulation plays a crucial role in tissue inflammatory reactions associated with helminth parasitic nfections and allergic diseases. Paragonimus westermani, a lung fluke causing human paragonimiasis, secretes a large amount of cysteine proteases, which are involved in nutrient uptake, tissue invasion, and modulation of hos's immune responses. There is, however, limited information about the response of eosinophils to direct stimulation by cysteine proteases (CP) secreted by P. westermani. In the present study, we tested whether degranulation and superoxide production from human eosinophils can be induced by stimulation of the 2 CP (27 kDa and 28 kDa) purified from excretory-secretory products (ESP) of P. westermani newly excysted metacercariae (PwNEM). A large quantity of eosinophil-derived neurotoxin (EDN) was detected in the culture supernatant when human eosinophils isolated from the peripheral blood were incubated with the purified 27 kDa CP. Furthermore, the 27 kDa CP induced superoxide anion production by eosinophils in time- and dose-dependent manners. In contrast, the purified 28 kDa CP did not induce superoxide production and degranulation. These findings suggest that the 27 kDa CP secreted by PwNEM induces superoxide production and degranulation of human eosinophils, which may be involved in eosinophil-mediated tissue inflammatory responses during the larval migration in human paragonimiasis-
dc.description.statementOfResponsibilityopen-
dc.format.extent95~99-
dc.relation.isPartOfKOREAN JOURNAL OF PARASITOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAstacoidea/parasitology-
dc.subject.MESHCell Degranulation*-
dc.subject.MESHCysteine Endopeptidases/immunology*-
dc.subject.MESHCysteine Endopeptidases/isolation & purification-
dc.subject.MESHEosinophils/immunology*-
dc.subject.MESHEosinophils/metabolism-
dc.subject.MESHHelminth Proteins/immunology*-
dc.subject.MESHHelminth Proteins/isolation & purification-
dc.subject.MESHHumans-
dc.subject.MESHParagonimiasis/immunology*-
dc.subject.MESHParagonimiasis/metabolism-
dc.subject.MESHParagonimus westermani/enzymology*-
dc.subject.MESHParagonimus westermani/isolation & purification-
dc.subject.MESHSuperoxides/immunology*-
dc.titleA 27 kDa Cysteine Protease Secreted by Newly Excysted Paragonimus westermani Metacercariae Induces Superoxide Anion Production and Degranulation of Human Eosinophils-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학)-
dc.contributor.googleauthorYoung-Bae Chung-
dc.contributor.googleauthorHirohito Kita-
dc.contributor.googleauthorMyeong Heon Shin-
dc.identifier.doi10.3347/kjp.2008.46.2.95-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02099-
dc.relation.journalcodeJ01957-
dc.identifier.eissn1738-0006-
dc.identifier.pmid18552546-
dc.subject.keywordParagonimus westermani-
dc.subject.keyword27 kDa cysteine protease-
dc.subject.keywordeosinophils-
dc.subject.keywordsuperoxide anion-
dc.subject.keyworddegranulation-
dc.contributor.alternativeNameShin, Myeong Heon-
dc.contributor.affiliatedAuthorShin, Myeong Heon-
dc.rights.accessRightsfree-
dc.citation.volume46-
dc.citation.number2-
dc.citation.startPage95-
dc.citation.endPage99-
dc.identifier.bibliographicCitationKOREAN JOURNAL OF PARASITOLOGY, Vol.46(2) : 95-99, 2008-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers

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