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Telomeric 3' overhangs in chronic HBV-related hepatitis and hepatocellular carcinoma

Authors
 Jae-Eun Lee  ;  Bong-Kyeong Oh  ;  Jinsub Choi  ;  Young Nyun Park 
Citation
 INTERNATIONAL JOURNAL OF CANCER, Vol.123(2) : 264-272, 2008 
Journal Title
 INTERNATIONAL JOURNAL OF CANCER 
ISSN
 0020-7136 
Issue Date
2008
MeSH
Adult ; Aged ; Carcinoma, Hepatocellular/enzymology ; Carcinoma, Hepatocellular/genetics* ; Carcinoma, Hepatocellular/virology ; Female ; Hepatitis B, Chronic/complications* ; Humans ; Keratin-19/analysis ; Liver Cirrhosis/genetics ; Liver Neoplasms/enzymology ; Liver Neoplasms/genetics* ; Liver Neoplasms/virology ; Male ; Middle Aged ; RNA, Messenger/analysis ; Telomerase/analysis* ; Telomerase/genetics ; Telomere* ; Telomere-Binding Proteins/analysis ; Telomeric Repeat Binding Protein 1/analysis
Keywords
telomeric 30overhang ; hepatocellular carcinoma ; telomere ; hTERT ; cytokeratin 19 ; hepatitis B virus
Abstract
Telomeric 3' overhang is a key component of telomere structure, but little is known about its role in hepatocarcinogenesis. We examined the 3' overhang and telomere length, mRNA levels of hTERT, POT1, TRF1 and cytokeratin 19 (CK19) in 41 hepatitis B virus (HBV)-related hepatocellular carcinomas (HCCs) and adjacent non-HCCs of B viral chronic hepatitis/cirrhosis. 3' overhang length was positively correlated with telomere length (p < 0.001). In non-HCCs, the 3' overhang shortened with increasing age (p = 0.043). Twenty-six HCCs had shorter and 15 HCCs had longer 3' overhangs than the adjacent non-HCCs. The mRNA levels of hTERT, POT1 and TRF1 were upregulated in HCCs than in non-HCCs. HCCs with lengthened 3' overhangs expressed higher hTERT mRNA levels than those with shortened 3' overhangs, when compared to 3' overhangs in non-HCCs (p = 0.044). POT1 and TRF1 showed no significant difference according to the 3' overhangs. HCCs with long 3' overhangs had higher mitosis (p = 0.046) and more frequent multipolar mitosis compared to those with short 3' overhangs (p = 0.034). HCCs with high cytokeratin 19 mRNA levels, a marker for hepatic progenitor cells, had longer 3' overhangs than HCCs with low cytokeratin 19 mRNA levels (p= 0.019). In conclusion, the 3' overhang erosion might be closely related to the number of cell divisions in telomerase-negative hepatocytes of chronic hepatitis/cirrhosis. In telomerase-positive HCCs, an altered 3' overhang are involved in HBV-related hepatocarcinogenesis and hTERT might be involved in regulation of 3' overhang
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/ijc.23376/abstract
DOI
10.1002/ijc.23376
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Cancer Metastasis Research Center (암전이연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Park, Young Nyun(박영년) ORCID logo https://orcid.org/0000-0003-0357-7967
Oh, Bong Kyeong(오봉경)
Choi, Jin Sub(최진섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107133
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