1 133

Cited 16 times in

The effect of PLGA sphere diameter on rabbit mesenchymal stem cells in adipose tissue engineering

Authors
 Yu Suk Choi  ;  Si-Nae Park  ;  Hwal Suh 
Citation
 JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, Vol.19(5) : 2165-2171, 2008 
Journal Title
 JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE 
ISSN
 0957-4530 
Issue Date
2008
MeSH
Adipocytes/cytology ; Animals ; Azo Compounds/pharmacology ; Cell Differentiation ; Cell Proliferation ; Green Fluorescent Proteins/metabolism ; Hexosaminidases/chemistry ; Lactic Acid/chemistry* ; Mesenchymal Stromal Cells/cytology* ; Mice ; Mice, Nude ; Microscopy, Electron, Scanning ; Microspheres ; Polyglycolic Acid/chemistry* ; Rabbits ; Tissue Engineering/methods
Keywords
Adipose Tissue ; Green Fluorescence Protein ; Nude Mouse ; Adipogenic Differentiation ; PLLA Scaffold
Abstract
To investigate the effect of injectable PLGA sphere's diameter on adipose tissue engineering, rabbit mesenchymal stem cells were attached to various diameters of injectable PLGA spheres (<75; 75-100; 100-150; 150-200; and 200-250 microm). These five groups were cultured in adipogenic media for 2 weeks in vitro and injected into necks of nude mice. Prior to in vivo study, cell proliferation and adipogenic differentiation were determined by hexosaminidase assay and Oil red O staining after 2 weeks. Group C (100-150 microm) showed the highest adipogenic differentiation and the proliferation capacity of Group B (75-100 microm) was significantly higher than that of any other group. We harvested newly formed tissues from necks of nude mice after 1 and 4 weeks. Although PLGA spheres have not been degraded and there was no significant histological difference among various sizes of spheres after 1 week, well-organized fat pads (PLGA spheres were completely degraded) could be observed, and the histology of the 100-150 microm groups resembled that of native tissue after 4 weeks. Based on these experiments, we could conclude that the optimal size of PLGA spheres for adipogenesis was 100-150 microm
Full Text
http://link.springer.com/article/10.1007%2Fs10856-007-3320-2
DOI
10.1007/s10856-007-3320-2
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Si Nae(박시내)
Suh, Hwal(서활)
Choi, Yu Suk(최유석)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107102
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse