Cited 53 times in
MCP-1/CCR2 system is involved in high glucose-induced fibronectin and type IV collagen expression in cultured mesangial cells
DC Field | Value | Language |
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dc.contributor.author | 이정은 | - |
dc.contributor.author | 한대석 | - |
dc.contributor.author | 한승혁 | - |
dc.contributor.author | 강신욱 | - |
dc.contributor.author | 김건홍 | - |
dc.contributor.author | 김동기 | - |
dc.contributor.author | 문성진 | - |
dc.contributor.author | 유태현 | - |
dc.date.accessioned | 2015-05-19T16:48:44Z | - |
dc.date.available | 2015-05-19T16:48:44Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1931-857X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/106989 | - |
dc.description.abstract | Monocyte chemoattractant protein-1 (MCP-1) is a potent chemokine that plays an important role in the recruitment of macrophages. Although previous studies have demonstrated the importance of MCP-1 in the pathogenesis of diabetic nephropathy (DN) in terms of inflammation, the role of MCP-1 and its receptor (C-C chemokine receptor 2; CCR2) in extracellular matrix (ECM) accumulation under diabetic conditions has been largely unexplored. This study was undertaken to investigate the functional role of the MCP-1/CCR2 system in high glucose-induced ECM (fibronectin and type IV collagen) protein expression in cultured mesangial cells (MCs). Mouse MCs were exposed to medium containing 5.6 mM glucose (NG), NG+24.4 mM mannitol (NG+M), or 30 mM glucose (HG) with or without mutant MCP-1 (mMCP-1), CCR2 small interfering (si)RNA, or CCR2 inhibitor (RS102895). To examine the relationship between MCP-1 and transforming growth factor (TGF)-beta1, MCs were also treated with TGF-beta1 (2 ng/ml) with or without mMCP-1 or CCR2 siRNA. Transient transfection was performed with Lipofectamine 2000 for 24 h. Cell viability was determined by an MTT assay, mouse and human MCP-1 and TGF-beta1 levels by ELISA, and CCR2 and ECM protein expression by Western blotting. Transfections of mMCP-1 and CCR2 siRNA increased human MCP-1 levels and inhibited CCR2 expression, respectively. HG-induced ECM protein expression and TGF-beta1 levels were significantly attenuated by mMCP-1, CCR2 siRNA, and RS102895 (P < 0.05). MCP-1 directly increased ECM protein expression, and this increase was inhibited by an anti-TGF-beta1 antibody. In addition, TGF-beta1-induced ECM protein expression was significantly abrogated by the inhibition of the MCP-1/CCR2 system (P < 0.05). These results suggest that an interaction between the MCP-1/CCR2 system and TGF-beta1 may contribute to ECM accumulation in DN. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | F749~F757 | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Chemokine CCL2/genetics | - |
dc.subject.MESH | Chemokine CCL2/metabolism* | - |
dc.subject.MESH | Collagen Type IV/metabolism* | - |
dc.subject.MESH | Diabetic Nephropathies/metabolism | - |
dc.subject.MESH | Fibronectins/metabolism* | - |
dc.subject.MESH | Glucose/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mesangial Cells/metabolism* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Transgenic | - |
dc.subject.MESH | Mutation | - |
dc.subject.MESH | RNA, Small Interfering/genetics | - |
dc.subject.MESH | Receptors, CCR2/genetics | - |
dc.subject.MESH | Receptors, CCR2/metabolism* | - |
dc.subject.MESH | Transfection | - |
dc.subject.MESH | Transforming Growth Factor beta1/metabolism | - |
dc.title | MCP-1/CCR2 system is involved in high glucose-induced fibronectin and type IV collagen expression in cultured mesangial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Jehyun Park | - |
dc.contributor.googleauthor | Dong-Ryeol Ryu | - |
dc.contributor.googleauthor | Jin Ji Li | - |
dc.contributor.googleauthor | Dong-Sub Jung | - |
dc.contributor.googleauthor | Seung-Jae Kwak | - |
dc.contributor.googleauthor | Sun Ha Lee | - |
dc.contributor.googleauthor | Tae-Hyun Yoo | - |
dc.contributor.googleauthor | Seung Hyeok Han | - |
dc.contributor.googleauthor | Jung Eun Lee | - |
dc.contributor.googleauthor | Dong Ki Kim | - |
dc.contributor.googleauthor | Sung Jin Moon | - |
dc.contributor.googleauthor | Kunhong Kim | - |
dc.contributor.googleauthor | Dae Suk Han | - |
dc.contributor.googleauthor | Shin-Wook Kang | - |
dc.identifier.doi | 10.1152/ajprenal.00547.2007 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04272 | - |
dc.contributor.localId | A04304 | - |
dc.contributor.localId | A00053 | - |
dc.contributor.localId | A00289 | - |
dc.contributor.localId | A01364 | - |
dc.contributor.localId | A02526 | - |
dc.contributor.localId | A03119 | - |
dc.contributor.localId | A00400 | - |
dc.relation.journalcode | J00108 | - |
dc.identifier.eissn | 1522-1466 | - |
dc.identifier.pmid | 18579703 | - |
dc.subject.keyword | diabetic nephropathy | - |
dc.subject.keyword | monocyte chemoattractant protein-1 | - |
dc.subject.keyword | transforming growth factor-β1 | - |
dc.subject.keyword | extracellular matrix | - |
dc.contributor.alternativeName | Lee, Jung Eun | - |
dc.contributor.alternativeName | Han, Dae Suk | - |
dc.contributor.alternativeName | Han, Seung Hyeok | - |
dc.contributor.alternativeName | Kang, Shin Wook | - |
dc.contributor.alternativeName | Kim, Kun Hong | - |
dc.contributor.alternativeName | Kim, Dong Ki | - |
dc.contributor.alternativeName | Moon, Sung Jin | - |
dc.contributor.alternativeName | Yoo, Tae Hyun | - |
dc.contributor.affiliatedAuthor | Han, Dae Suk | - |
dc.contributor.affiliatedAuthor | Han, Seung Hyeok | - |
dc.contributor.affiliatedAuthor | Kang, Shin Wook | - |
dc.contributor.affiliatedAuthor | Kim, Kun Hong | - |
dc.contributor.affiliatedAuthor | Moon, Sung Jin | - |
dc.contributor.affiliatedAuthor | Yoo, Tae Hyun | - |
dc.contributor.affiliatedAuthor | Lee, Jung Eun | - |
dc.contributor.affiliatedAuthor | Kim, Dong Ki | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 295 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 749 | - |
dc.citation.endPage | 757 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, Vol.295(3) : 749-757, 2008 | - |
dc.identifier.rimsid | 56415 | - |
dc.type.rims | ART | - |
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