Cited 14 times in
The AdLMP-1 transfection in two different cells; AF cells, chondrocytes as potential cell therapy candidates for disc degeneration.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 구성욱 | - |
dc.date.accessioned | 2015-05-19T16:44:55Z | - |
dc.date.available | 2015-05-19T16:44:55Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0001-6268 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/106871 | - |
dc.description.abstract | BACKGROUND: LMP-1 is known to increase proteoglycan production through the upregulating the BMPs and it is also known that BMP-2 acts on anulus fibrosus cells and chondrocytes to increase proteoglycan production. METHOD: We carried out an experiment, the effect of AdLMP-1 transfection on AF cells and chondrocytes in the production of sulfated-glycosaminoglycans, mRNA expression (aggrecan, type I, II collagen, LMP-1, BMP-2, and BMP-7), and immunofluorescence staining. AF cells and chondrocytes were grown in monolayer and treated for 6 days with AdLMP1-green fluorescence protein (GFP) (10, 20, and 30 multiplicity of infection [MOI]). After 6 days, the sGAG content in the media was quantified using 1,9-dimethylmethylene blue staining. The mRNA expression was measured with real-time PCR after 20 MOI infection of AdLMP1-GFP. The each cells treated with 20 MOI infection of AdGFP was used as a control group for the mRNA expression. The each cell group was immunofluorescence stained with each antibodies in the chamber slide at 3 x 10(4) cells/chamber. FINDINGS: 1) The sGAG production was maximum in 20 MOI AdLMP1-GFP infection on the AdLMP-1 treatment for both of AF cells and chondrocytes. 2) The mRNA expression of aggrecan, type I collagen, type II collagen, LMP-1, BMP-2, and BMP-7 is increased in both AF cells and chondrocytes in 20 MOI AdLMP1-GFP infection. 3) On the immunofluorescence staining results, the positive immunofluorescence stained cell numbers are increased after 20 MOI AdLMP1-GFP infection concordant with upregulation of mRNA expression. CONCLUSIONS: The AdLMP-1 treatments in AF cells and chondrocytes may be useful for cell transplantation therapy in disc degeneration | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 803~810 | - |
dc.relation.isPartOf | ACTA NEUROCHIRURGICA | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adaptor Proteins, Signal Transducing | - |
dc.subject.MESH | Aggrecans/genetics | - |
dc.subject.MESH | Bone Morphogenetic Protein 2 | - |
dc.subject.MESH | Bone Morphogenetic Protein 7 | - |
dc.subject.MESH | Bone Morphogenetic Proteins/metabolism | - |
dc.subject.MESH | Cell Transplantation* | - |
dc.subject.MESH | Chondrocytes/metabolism* | - |
dc.subject.MESH | Chondrocytes/pathology | - |
dc.subject.MESH | Chondrocytes/transplantation* | - |
dc.subject.MESH | Collagen Type I/genetics | - |
dc.subject.MESH | Collagen Type II/genetics | - |
dc.subject.MESH | Cytoskeletal Proteins | - |
dc.subject.MESH | Gene Expression Regulation/genetics | - |
dc.subject.MESH | Genetic Therapy/methods* | - |
dc.subject.MESH | Glycosaminoglycans/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Intervertebral Disc/metabolism* | - |
dc.subject.MESH | Intervertebral Disc/pathology | - |
dc.subject.MESH | Intracellular Signaling Peptides and Proteins/genetics* | - |
dc.subject.MESH | LIM Domain Proteins | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | Spinal Diseases/therapy* | - |
dc.subject.MESH | Transfection/methods* | - |
dc.subject.MESH | Transforming Growth Factor beta/metabolism | - |
dc.title | The AdLMP-1 transfection in two different cells; AF cells, chondrocytes as potential cell therapy candidates for disc degeneration. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurosurgery (신경외과학) | - |
dc.contributor.googleauthor | Kuh SU | - |
dc.contributor.googleauthor | Zhu Y | - |
dc.contributor.googleauthor | Li J | - |
dc.contributor.googleauthor | Tsai KJ | - |
dc.contributor.googleauthor | Fei Q | - |
dc.contributor.googleauthor | Hutton WC | - |
dc.contributor.googleauthor | Yoon ST | - |
dc.identifier.doi | 10.1007/s00701-008-1617-7 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00196 | - |
dc.relation.journalcode | J00018 | - |
dc.identifier.eissn | 0942-0940 | - |
dc.identifier.pmid | 18618069 | - |
dc.identifier.url | http://link.springer.com/article/10.1007/s00701-008-1617-7 | - |
dc.subject.keyword | Proteoglycan | - |
dc.subject.keyword | Anulus fibrosus cell | - |
dc.subject.keyword | Chondrocyte | - |
dc.subject.keyword | AdLMP-1 | - |
dc.contributor.alternativeName | Kuh, Sung Uk | - |
dc.contributor.affiliatedAuthor | Kuh, Sung Uk | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 150 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 803 | - |
dc.citation.endPage | 810 | - |
dc.identifier.bibliographicCitation | ACTA NEUROCHIRURGICA, Vol.150(8) : 803-810, 2008 | - |
dc.identifier.rimsid | 50901 | - |
dc.type.rims | ART | - |
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