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The role of nerve growth factor in hyperosmolar stress induced apoptosis

Authors
 EUN-JU CHANG  ;  YOUNG SUN IM  ;  EUNDUCK P. KAY  ;  JONG YOUL KIM  ;  JONG EUN LEE  ;  HYUNG KEUN LEE 
Citation
 JOURNAL OF CELLULAR PHYSIOLOGY, Vol.216(1) : 69-77, 2008 
Journal Title
 JOURNAL OF CELLULAR PHYSIOLOGY 
ISSN
 0021-9541 
Issue Date
2008
MeSH
Animals ; Apoptosis/physiology* ; Cornea/cytology* ; Enzyme Inhibitors/metabolism ; Epithelial Cells/cytology ; Epithelial Cells/physiology* ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Gene Expression Regulation ; Humans ; Interleukin-1beta/genetics ; Interleukin-1beta/metabolism ; JNK Mitogen-Activated Protein Kinases/genetics ; JNK Mitogen-Activated Protein Kinases/metabolism ; MAP Kinase Signaling System/physiology ; NF-kappa B/genetics ; NF-kappa B/metabolism ; Nerve Growth Factor/genetics ; Nerve Growth Factor/metabolism* ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/metabolism ; Osmolar Concentration* ; Phosphorylation ; Rats ; Rats, Sprague-Dawley ; Receptor, trkA/genetics ; Receptor, trkA/metabolism ; Receptors, Nerve Growth Factor/genetics ; Receptors, Nerve Growth Factor/metabolism ; Transcription Factor AP-1/genetics ; Transcription Factor AP-1/metabolism ; Tumor Necrosis Factor-alpha/genetics ; Tumor Necrosis Factor-alpha/metabolism
Keywords
Animals ; Apoptosis/physiology* ; Cornea/cytology* ; Enzyme Inhibitors/metabolism ; Epithelial Cells/cytology ; Epithelial Cells/physiology* ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Gene Expression Regulation ; Humans ; Interleukin-1beta/genetics ; Interleukin-1beta/metabolism ; JNK Mitogen-Activated Protein Kinases/genetics ; JNK Mitogen-Activated Protein Kinases/metabolism ; MAP Kinase Signaling System/physiology ; NF-kappa B/genetics ; NF-kappa B/metabolism ; Nerve Growth Factor/genetics ; Nerve Growth Factor/metabolism* ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/metabolism ; Osmolar Concentration* ; Phosphorylation ; Rats ; Rats, Sprague-Dawley ; Receptor, trkA/genetics ; Receptor, trkA/metabolism ; Receptors, Nerve Growth Factor/genetics ; Receptors, Nerve Growth Factor/metabolism ; Transcription Factor AP-1/genetics ; Transcription Factor AP-1/metabolism ; Tumor Necrosis Factor-alpha/genetics ; Tumor Necrosis Factor-alpha/metabolism
Abstract
Nerve growth factor (NGF) is a neurotrophic factor that plays an important role in the differentiation and growth of neuronal cells. It is also regarded as an inflammatory mediator in non-neuronal tissues under physiological stress conditions. The mechanisms of NGF production and its roles in hyperosmolar stress conditions have not been established. In this study, we show that NGF levels in cultured human corneal epithelial cells (HCECs) were up-regulated during hyperosmolar stress by IL-1beta, but not TNF-alpha. NF-kappaB activity, but not AP-1, increased significantly under hyperosmolar conditions, and NF-kappaB was involved in IL-1beta-induced NGF production. IL-1beta-induced NGF production reduced JNK phosphorylation and HCEC apoptosis. These changes were accompanied by down-regulated Bax and caspase-3, -8, -9 activities. NGF siRNA and the tyrosine kinase inhibitor K252a significantly enhanced Bax up-regulation. Thus, up-regulated NGF under hyperosmolar stress conditions may contribute, at least in part, to reduced HCEC apoptosis. This conclusion suggests that enhanced NGF expression may be beneficial in recovering corneal damage due to chronic hyperosmolar stress
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jcp.21377/abstract
DOI
10.1002/jcp.21377
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jong Youl(김종열) ORCID logo https://orcid.org/0000-0002-8340-2894
Lee, Jong Eun(이종은) ORCID logo https://orcid.org/0000-0001-6203-7413
Lee, Hyung Keun(이형근) ORCID logo https://orcid.org/0000-0002-1123-2136
Im, Young Sun(임영선)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/106548
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