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Induction of cell death in human macrophages by a highly virulent Korean Isolate of Mycobacterium tuberculosis and the virulent strain H37Rv.

Authors
 H. Sohn  ;  K.-S. Lee  ;  S.-Y. Kim  ;  D.-M. Shin  ;  S.-J. Shin  ;  E.-K. Jo  ;  J.-K. Park  ;  H.-J. Kim 
Citation
 SCANDINAVIAN JOURNAL OF IMMUNOLOGY, Vol.69(1) : 43-50, 2009 
Journal Title
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
ISSN
 0300-9475 
Issue Date
2009
MeSH
Apoptosis/immunology* ; Cell Line ; Cell Survival ; Cytokines/metabolism ; Humans ; Korea ; Macrophages/immunology* ; Macrophages/microbiology ; Minor Histocompatibility Antigens ; Mycobacterium tuberculosis/pathogenicity* ; Myeloid Cell Leukemia Sequence 1 Protein ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Tuberculosis/immunology* ; Tuberculosis/microbiology ; Up-Regulation ; Virulence ; bcl-2-Associated X Protein/metabolism ; bcl-X Protein/metabolism
Abstract
Recent studies have suggested that virulent strains of Mycobacterium tuberculosis induce apoptosis in macrophages less often than do attenuated strains. K-strain, which belongs to the Beijing family, is the most frequently isolated clinical strain of M. tuberculosis in Korea. In this study, we investigated the differential induction of cell death in human monocytic THP-1 cells by K-strain and H37Rv, a virulent but laboratory-adapted strain of M. tuberculosis. Although no significant difference in growth rate was observed between the cells exposed to K-strain and those exposed to H37Rv, the levels of protective cytokines such as tumour necrosis factor (TNF)-alpha, interleukin (IL)-6 and IL-12p40 were lower in K-strain-infected cells than in H37Rv-infected cells. Cell viability assays showed that both K-strain and H37Rv, but not heat- or streptomycin-killed bacteria, induced THP-1 cell death in a TNF-independent manner. In contrast, double staining with fluorochrome-labelled inhibitors of caspase and propidium iodide and lactate dehydrogenase release assays revealed that K-strain induced significantly higher levels of necrotic cell death, rather than apoptosis, in THP-1 cells than did H37Rv. Anti-apoptotic Bcl-2, Mcl-1, Bfl-1 and Bcl-xL in the cells were significantly upregulated following infection with K-strain compared with H37Rv, whereas Bax was slightly upregulated in response to infection with both H37Rv and K-strain. These results suggest that the highly virulent K-strain keeps cellular apoptosis as a host defense mechanism to a minimum and induces necrosis in macrophages
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3083.2008.02188.x/abstract
DOI
10.1111/j.1365-3083.2008.02188.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Shin, Sung Jae(신성재) ORCID logo https://orcid.org/0000-0003-0854-4582
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/106041
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