622 492

Cited 0 times in

간섬유화가 유도된 백서에서 이식된 간세포 착상과 증식에 관여하는 인자

DC Field Value Language
dc.contributor.author김경식-
dc.date.accessioned2015-04-24T17:40:23Z-
dc.date.available2015-04-24T17:40:23Z-
dc.date.issued2009-
dc.identifier.issn1738-6349-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/105847-
dc.description.abstractPurpose: Cell therapy for various diseases has gained wide acceptance. Because most patients with chronic liver failure have mild-to-severe liver cirrhosis, there are many limitations to clinical applications. We analyzed how to increase cell engraftment in rats with liver fibrosis. Methods: We used analbuminemic SD rats (NARs) as recipients of syngeneic CAG-EGFP SD hepatocytes obtained by the 2 perfusion method. Hepatic fibrosis was induced with thioacetamide in drinking water for 6 weeks in the recipient NARs. NARs were pre-treated with gadolinium, doxorubicin, and gliotoxin before hepatocyte transplantation. We evaluated the degree of cell engraftment by RT-PCR and immunofluorescent staining for GFP and albumin. The transplanted cells were detected by immunostaining for albumin, and serum albumin was also measured. Results: Although detection of GFP by RT-PCR was variable, albumin was detected in all groups 4 wks after hepatocyte transplantation. GFP and albumin were also detected by immunofluorescent staining 1 and 4 wks after cell transplantation. In control rats, albumin production was maximal at 3 wks, and after that it rapidly decreased. In the gadolinium and doxorubicin-treated group, albumin production was increased up to 4 wks. Albumin production in the gadolinium-treated group was superior to that of the doxorubicin-treated group. Conclusion: Kupffer cells play the most important role in cell engraftment in hepatic fibrosis. Therefore, perturbation of kupffer cells in hepatic fibrosis is needed to increase cell engraftment-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageKorean Journal of Hepato-Biliary-Pancreatic Surgery-
dc.publisherKorean Journal of Hepato-Biliary-Pancreatic Surgery-
dc.relation.isPartOfKorean Journal of Hepato-Biliary-Pancreatic Surgery-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.title간섬유화가 유도된 백서에서 이식된 간세포 착상과 증식에 관여하는 인자-
dc.title.alternativeThe Factors Affecting Transplanted Hepatocytes Repopulation in Rats with Liver Fibrosis-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Surgery (외과학)-
dc.contributor.googleauthor이상우-
dc.contributor.googleauthor민선옥-
dc.contributor.googleauthor최새별-
dc.contributor.googleauthor김경식-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00299-
dc.contributor.localIdA04069-
dc.relation.journalcodeJ02035-
dc.identifier.eissn2288-9213-
dc.subject.keywordHepatocyte transplantation-
dc.subject.keywordEngraftment-
dc.subject.keywordLiver fibrosis-
dc.subject.keywordKupffer cell-
dc.subject.keywordAnalbuminemic rat (NAR)-
dc.contributor.alternativeNameKim, Kyung Sik-
dc.contributor.affiliatedAuthorKim, Kyung Sik-
dc.citation.volume13-
dc.citation.number4-
dc.citation.startPage189-
dc.citation.endPage197-
dc.identifier.bibliographicCitationKorean Journal of Hepato-Biliary-Pancreatic Surgery, Vol.13(4) : 189-197, 2009-
dc.identifier.rimsid49884-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.