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간섬유화가 유도된 백서에서 이식된 간세포 착상과 증식에 관여하는 인자
DC Field | Value | Language |
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dc.contributor.author | 김경식 | - |
dc.date.accessioned | 2015-04-24T17:40:23Z | - |
dc.date.available | 2015-04-24T17:40:23Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1738-6349 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/105847 | - |
dc.description.abstract | Purpose: Cell therapy for various diseases has gained wide acceptance. Because most patients with chronic liver failure have mild-to-severe liver cirrhosis, there are many limitations to clinical applications. We analyzed how to increase cell engraftment in rats with liver fibrosis. Methods: We used analbuminemic SD rats (NARs) as recipients of syngeneic CAG-EGFP SD hepatocytes obtained by the 2 perfusion method. Hepatic fibrosis was induced with thioacetamide in drinking water for 6 weeks in the recipient NARs. NARs were pre-treated with gadolinium, doxorubicin, and gliotoxin before hepatocyte transplantation. We evaluated the degree of cell engraftment by RT-PCR and immunofluorescent staining for GFP and albumin. The transplanted cells were detected by immunostaining for albumin, and serum albumin was also measured. Results: Although detection of GFP by RT-PCR was variable, albumin was detected in all groups 4 wks after hepatocyte transplantation. GFP and albumin were also detected by immunofluorescent staining 1 and 4 wks after cell transplantation. In control rats, albumin production was maximal at 3 wks, and after that it rapidly decreased. In the gadolinium and doxorubicin-treated group, albumin production was increased up to 4 wks. Albumin production in the gadolinium-treated group was superior to that of the doxorubicin-treated group. Conclusion: Kupffer cells play the most important role in cell engraftment in hepatic fibrosis. Therefore, perturbation of kupffer cells in hepatic fibrosis is needed to increase cell engraftment | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | Korean Journal of Hepato-Biliary-Pancreatic Surgery | - |
dc.publisher | Korean Journal of Hepato-Biliary-Pancreatic Surgery | - |
dc.relation.isPartOf | Korean Journal of Hepato-Biliary-Pancreatic Surgery | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 간섬유화가 유도된 백서에서 이식된 간세포 착상과 증식에 관여하는 인자 | - |
dc.title.alternative | The Factors Affecting Transplanted Hepatocytes Repopulation in Rats with Liver Fibrosis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | 이상우 | - |
dc.contributor.googleauthor | 민선옥 | - |
dc.contributor.googleauthor | 최새별 | - |
dc.contributor.googleauthor | 김경식 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00299 | - |
dc.contributor.localId | A04069 | - |
dc.relation.journalcode | J02035 | - |
dc.identifier.eissn | 2288-9213 | - |
dc.subject.keyword | Hepatocyte transplantation | - |
dc.subject.keyword | Engraftment | - |
dc.subject.keyword | Liver fibrosis | - |
dc.subject.keyword | Kupffer cell | - |
dc.subject.keyword | Analbuminemic rat (NAR) | - |
dc.contributor.alternativeName | Kim, Kyung Sik | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Sik | - |
dc.citation.volume | 13 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 189 | - |
dc.citation.endPage | 197 | - |
dc.identifier.bibliographicCitation | Korean Journal of Hepato-Biliary-Pancreatic Surgery, Vol.13(4) : 189-197, 2009 | - |
dc.identifier.rimsid | 49884 | - |
dc.type.rims | ART | - |
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