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Analysis of interleukin-21-induced Prdm1 gene regulation reveals functional cooperation of STAT3 and IRF4 transcription factors

DC Field Value Language
dc.contributor.author김형표-
dc.date.accessioned2015-04-24T17:38:33Z-
dc.date.available2015-04-24T17:38:33Z-
dc.date.issued2009-
dc.identifier.issn1074-7613-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/105789-
dc.description.abstractInterleukin-21 (IL-21) is a pleiotropic cytokine that induces expression of transcription factor BLIMP1 (encoded by Prdm1), which regulates plasma cell differentiation and T cell homeostasis. We identified an IL-21 response element downstream of Prdm1 that binds the transcription factors STAT3 and IRF4, which are required for optimal Prdm1 expression. Genome-wide ChIP-Seq mapping of STAT3- and IRF4-binding sites showed that most regions with IL-21-induced STAT3 binding also bound IRF4 in vivo and furthermore revealed that the noncanonical TTCnnnTAA GAS motif critical in Prdm1 was broadly used for STAT3 binding. Comparing genome-wide expression array data to binding sites revealed that most IL-21-regulated genes were associated with combined STAT3-IRF4 sites rather than pure STAT3 sites. Correspondingly, ChIP-Seq analysis of Irf4(-/-) T cells showed greatly diminished STAT3 binding after IL-21 treatment, and Irf4(-/-) mice showed impaired IL-21-induced Tfh cell differentiation in vivo. These results reveal broad cooperative gene regulation by STAT3 and IRF4.-
dc.description.statementOfResponsibilityopen-
dc.format.extent941~952-
dc.relation.isPartOfIMMUNITY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHB-Lymphocytes/immunology-
dc.subject.MESHBase Sequence-
dc.subject.MESHBinding Sites-
dc.subject.MESHCD4-Positive T-Lymphocytes/immunology-
dc.subject.MESHCell Differentiation-
dc.subject.MESHGene Expression Regulation*-
dc.subject.MESHGenome-Wide Association Study-
dc.subject.MESHInterferon Regulatory Factors/genetics-
dc.subject.MESHInterferon Regulatory Factors/metabolism*-
dc.subject.MESHInterleukins/metabolism*-
dc.subject.MESHIntrons-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHMice, Knockout-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHPositive Regulatory Domain I-Binding Factor 1-
dc.subject.MESHSTAT3 Transcription Factor/genetics-
dc.subject.MESHSTAT3 Transcription Factor/metabolism*-
dc.subject.MESHTranscription Factors/genetics*-
dc.titleAnalysis of interleukin-21-induced Prdm1 gene regulation reveals functional cooperation of STAT3 and IRF4 transcription factors-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학)-
dc.contributor.googleauthorHyokjoon Kwon-
dc.contributor.googleauthorDanielle Thierry-Mieg-
dc.contributor.googleauthorJean Thierry-Mieg-
dc.contributor.googleauthorHyoung-Pyo Kim-
dc.contributor.googleauthorJangsuk Oh-
dc.contributor.googleauthorChainarong Tunyaplin-
dc.contributor.googleauthorSebastian Carotta-
dc.contributor.googleauthorColleen E. Donovan-
dc.contributor.googleauthorMatthew L.Goldman-
dc.contributor.googleauthorPrafullakumar Tailor-
dc.contributor.googleauthorKeiko Ozato-
dc.contributor.googleauthorDavid E. Levy-
dc.contributor.googleauthorStephen L. Nutt-
dc.contributor.googleauthorKathryn Calame-
dc.contributor.googleauthorWarren J. Leonard-
dc.identifier.doi10.1016/j.immuni.2009.10.008-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01163-
dc.relation.journalcodeJ01034-
dc.identifier.eissn1097-4180-
dc.identifier.pmid20064451-
dc.contributor.alternativeNameKim, Hyoung Pyo-
dc.contributor.affiliatedAuthorKim, Hyoung Pyo-
dc.citation.volume31-
dc.citation.number6-
dc.citation.startPage941-
dc.citation.endPage952-
dc.identifier.bibliographicCitationIMMUNITY, Vol.31(6) : 941-952, 2009-
dc.identifier.rimsid49849-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers

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