Cited 21 times in
Upregulation of MUC5AC gene expression by IL-4 through CREB in human airway epithelial cells
DC Field | Value | Language |
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dc.contributor.author | 김규언 | - |
dc.contributor.author | 윤주헌 | - |
dc.date.accessioned | 2015-04-24T17:32:20Z | - |
dc.date.available | 2015-04-24T17:32:20Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0730-2312 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/105590 | - |
dc.description.abstract | Mucus hypersecretion is an important characteristic feature of the pathogenesis of allergy. Although interleukin (IL)-4 is known to be an inflammatory mediator in respiratory diseases, the mechanism by which IL-4 induces MUC5AC gene expression has not been fully explored. The aim of this study was to investigate the mechanism by which IL-4 induces MUC5AC gene expression in the airway. We examined the role of mitogen-activated protein kinase (MAPK) signaling on MUC5AC gene expression in airway epithelium. We showed that phosphorylation of ERK1/2 increased after treatment of cells with IL-4, whereas phosphorylation of p38 and JNK was not detected. In addition, pharmacologic and genetic inhibition of ERK1/2 abolished IL-4-induced MUC5AC gene expression. Moreover, we investigated the activation of p90 ribosomal S6 kinase 1 (RSK1) as a downstream signaling target of ERK1/2 in IL-4 signaling. The activation of RSK1 was prevented by pretreatment with PD98059 or plasmid expressing a MEK1 dominant-negative mutant. We also found that RSK1 mediated the IL-4-induced phosphorylation of cAMP response element-binding protein (CREB) and the transcription of MUC5AC. Furthermore, the cAMP-response element (CRE) in the MUC5AC promoter appears to be important for IL-4-induced MUC5AC gene expression in NCI-H292 cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 974~981 | - |
dc.relation.isPartOf | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cyclic AMP/metabolism | - |
dc.subject.MESH | Cyclic AMP Response Element-Binding Protein/metabolism* | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Enzyme Inhibitors/pharmacology | - |
dc.subject.MESH | Epithelial Cells/metabolism* | - |
dc.subject.MESH | Extracellular Signal-Regulated MAP Kinases/metabolism | - |
dc.subject.MESH | Flavonoids/pharmacology | - |
dc.subject.MESH | Gene Expression Regulation* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-4/metabolism* | - |
dc.subject.MESH | Mucin 5AC/biosynthesis* | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Response Elements/genetics | - |
dc.subject.MESH | Up-Regulation | - |
dc.subject.MESH | p38 Mitogen-Activated Protein Kinases/metabolism | - |
dc.title | Upregulation of MUC5AC gene expression by IL-4 through CREB in human airway epithelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pediatrics (소아과학) | - |
dc.contributor.googleauthor | Cheol Hong Kim | - |
dc.contributor.googleauthor | Kyu-Earn Kim | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.contributor.googleauthor | Kyoung Seob Song | - |
dc.identifier.doi | 10.1002/jcb.22330 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00327 | - |
dc.contributor.localId | A02604 | - |
dc.relation.journalcode | J01303 | - |
dc.identifier.eissn | 1097-4644 | - |
dc.identifier.pmid | 19718656 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/jcb.22330/abstract | - |
dc.subject.keyword | allergen | - |
dc.subject.keyword | asthma | - |
dc.subject.keyword | IL‐4 | - |
dc.subject.keyword | MUC5AC | - |
dc.subject.keyword | CREB | - |
dc.contributor.alternativeName | Kim, Kyu Earn | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Kim, Kyu Earn | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.citation.volume | 108 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 974 | - |
dc.citation.endPage | 981 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELLULAR BIOCHEMISTRY, Vol.108(4) : 974-981, 2009 | - |
dc.identifier.rimsid | 40901 | - |
dc.type.rims | ART | - |
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