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Characterization of a new metallo-beta-lactamase gene, bla(NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India

DC FieldValueLanguage
dc.contributor.author용동은-
dc.contributor.author이경원-
dc.date.accessioned2015-04-24T17:26:37Z-
dc.date.available2015-04-24T17:26:37Z-
dc.date.issued2009-
dc.identifier.issn0066-4804-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/105409-
dc.description.abstractA Swedish patient of Indian origin traveled to New Delhi, India, and acquired a urinary tract infection caused by a carbapenem-resistant Klebsiella pneumoniae strain that typed to the sequence type 14 complex. The isolate, Klebsiella pneumoniae 05-506, was shown to possess a metallo-beta-lactamase (MBL) but was negative for previously known MBL genes. Gene libraries and amplification of class 1 integrons revealed three resistance-conferring regions; the first contained bla(CMY-4) flanked by ISEcP1 and blc. The second region of 4.8 kb contained a complex class 1 integron with the gene cassettes arr-2, a new erythromycin esterase gene; ereC; aadA1; and cmlA7. An intact ISCR1 element was shown to be downstream from the qac/sul genes. The third region consisted of a new MBL gene, designated bla(NDM-1), flanked on one side by K. pneumoniae DNA and a truncated IS26 element on its other side. The last two regions lie adjacent to one another, and all three regions are found on a 180-kb region that is easily transferable to recipient strains and that confers resistance to all antibiotics except fluoroquinolones and colistin. NDM-1 shares very little identity with other MBLs, with the most similar MBLs being VIM-1/VIM-2, with which it has only 32.4% identity. As well as possessing unique residues near the active site, NDM-1 also has an additional insert between positions 162 and 166 not present in other MBLs. NDM-1 has a molecular mass of 28 kDa, is monomeric, and can hydrolyze all beta-lactams except aztreonam. Compared to VIM-2, NDM-1 displays tighter binding to most cephalosporins, in particular, cefuroxime, cefotaxime, and cephalothin (cefalotin), and also to the penicillins. NDM-1 does not bind to the carbapenems as tightly as IMP-1 or VIM-2 and turns over the carbapenems at a rate similar to that of VIM-2. In addition to K. pneumoniae 05-506, bla(NDM-1) was found on a 140-kb plasmid in an Escherichia coli strain isolated from the patient's feces, inferring the possibility of in vivo conjugation. The broad resistance carried on these plasmids is a further worrying development for India, which already has high levels of antibiotic resistance-
dc.description.statementOfResponsibilityopen-
dc.format.extent5046~5054-
dc.relation.isPartOfANTIMICROBIAL AGENTS AND CHEMOTHERAPY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHAnti-Bacterial Agents/metabolism-
dc.subject.MESHAnti-Bacterial Agents/pharmacology-
dc.subject.MESHCarboxylic Ester Hydrolases/chemistry*-
dc.subject.MESHCarboxylic Ester Hydrolases/classification-
dc.subject.MESHCarboxylic Ester Hydrolases/genetics*-
dc.subject.MESHCefotaxime/metabolism-
dc.subject.MESHCefotaxime/pharmacology-
dc.subject.MESHCefuroxime/metabolism-
dc.subject.MESHCefuroxime/pharmacology-
dc.subject.MESHCephalosporins/metabolism-
dc.subject.MESHCephalosporins/pharmacology-
dc.subject.MESHCephalothin/metabolism-
dc.subject.MESHCephalothin/pharmacology-
dc.subject.MESHDrug Resistance, Multiple, Bacterial/genetics-
dc.subject.MESHDrug Resistance, Multiple, Bacterial/physiology-
dc.subject.MESHElectrophoresis, Gel, Pulsed-Field-
dc.subject.MESHHumans-
dc.subject.MESHIndia-
dc.subject.MESHKinetics-
dc.subject.MESHKlebsiella Infections/microbiology*-
dc.subject.MESHKlebsiella pneumoniae/drug effects-
dc.subject.MESHKlebsiella pneumoniae/enzymology*-
dc.subject.MESHKlebsiella pneumoniae/genetics*-
dc.subject.MESHMicrobial Sensitivity Tests-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHPenicillins/metabolism-
dc.subject.MESHPenicillins/pharmacology-
dc.subject.MESHSequence Analysis, DNA-
dc.subject.MESHSequence Homology, Amino Acid-
dc.subject.MESHbeta-Lactamases/chemistry*-
dc.subject.MESHbeta-Lactamases/classification-
dc.subject.MESHbeta-Lactamases/genetics*-
dc.titleCharacterization of a new metallo-beta-lactamase gene, bla(NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorDongeun Yong-
dc.contributor.googleauthorMark A. Toleman-
dc.contributor.googleauthorChristian G. Giske-
dc.contributor.googleauthorHyun S. Cho-
dc.contributor.googleauthorKristina Sundman-
dc.contributor.googleauthorKyungwon Lee-
dc.contributor.googleauthorTimothy R. Walsh-
dc.identifier.doi10.1128/AAC.00774-09-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02423-
dc.contributor.localIdA02649-
dc.relation.journalcodeJ00189-
dc.identifier.eissn1098-6596-
dc.identifier.pmid19770275-
dc.contributor.alternativeNameYong, Dong Eun-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.affiliatedAuthorYong, Dong Eun-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.citation.volume53-
dc.citation.number12-
dc.citation.startPage5046-
dc.citation.endPage5054-
dc.identifier.bibliographicCitationANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Vol.53(12) : 5046-5054, 2009-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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