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Broth microdilution method to detect extended-spectrum beta-lactamases and AmpC beta-lactamases in enterobacteriaceae isolates by use of clavulanic acid and boronic acid as inhibitors

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dc.contributor.author정석훈-
dc.date.accessioned2015-04-24T17:22:53Z-
dc.date.available2015-04-24T17:22:53Z-
dc.date.issued2009-
dc.identifier.issn0095-1137-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/105292-
dc.description.abstractThis study was designed to evaluate the performance of the broth microdilution (BMD) method to detect production of extended-spectrum beta-lactamases (ESBLs) and AmpC beta-lactamases in Enterobacteriaceae by using clavulanic acid (CA) and boronic acid (BA) as ESBL and AmpC beta-lactamase inhibitors, respectively. A total of 100 clinical isolates of Enterobacteriaceae were analyzed. Mueller-Hinton broth containing serial twofold dilutions of cefotaxime (CTX), ceftazidime (CAZ), aztreonam (ATM), or cefepime (FEP) with or without either or both CA and BA was prepared. An eightfold or greater decrease in the MIC of CTX, CAZ, ATM, or FEP in the presence of CA and BA was considered a positive result for ESBL and plasmid-mediated AmpC beta-lactamase (PABL), respectively. In tests with CA, expanded-spectrum beta-lactams containing BA (CTX-BA, CAZ-BA, ATM-BA, and FEP-BA) showed higher positive rates in detecting ESBL producers than those without BA. The combination of CTX- and CAZ-based BMD tests with CA and BA showed sensitivity and specificity of 100% for the detection of ESBLs and PABLs. The BMD testing could be applicable for routine use in commercially available semiautomated systems for the detection of ESBLs and PABLs in Enterobacteriaceae.-
dc.description.statementOfResponsibilityopen-
dc.format.extent3409~3412-
dc.relation.isPartOfJOURNAL OF CLINICAL MICROBIOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnti-Bacterial Agents/pharmacology-
dc.subject.MESHBoronic Acids/pharmacology*-
dc.subject.MESHClavulanic Acid/pharmacology*-
dc.subject.MESHCulture Media/chemistry-
dc.subject.MESHEnterobacteriaceae/drug effects*-
dc.subject.MESHEnterobacteriaceae/enzymology*-
dc.subject.MESHEnterobacteriaceae/isolation & purification-
dc.subject.MESHEnterobacteriaceae Infections/microbiology-
dc.subject.MESHEnzyme Inhibitors/pharmacology*-
dc.subject.MESHHumans-
dc.subject.MESHMicrobial Sensitivity Tests/methods*-
dc.subject.MESHSensitivity and Specificity-
dc.subject.MESHbeta-Lactamase Inhibitors-
dc.subject.MESHbeta-Lactamases/biosynthesis*-
dc.titleBroth microdilution method to detect extended-spectrum beta-lactamases and AmpC beta-lactamases in enterobacteriaceae isolates by use of clavulanic acid and boronic acid as inhibitors-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorSeok Hoon Jeong-
dc.contributor.googleauthorWonkeun Song-
dc.contributor.googleauthorJae-Seok Kim-
dc.contributor.googleauthorHan-Sung Kim-
dc.contributor.googleauthorKyu Man Lee-
dc.identifier.doi10.1128/JCM.01141-09-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03619-
dc.relation.journalcodeJ01325-
dc.identifier.eissn1098-660X-
dc.identifier.pmid19710269-
dc.contributor.alternativeNameJeong, Seok Hoon-
dc.contributor.affiliatedAuthorJeong, Seok Hoon-
dc.citation.volume47-
dc.citation.number11-
dc.citation.startPage3409-
dc.citation.endPage3412-
dc.identifier.bibliographicCitationJOURNAL OF CLINICAL MICROBIOLOGY, Vol.47(11) : 3409-3412, 2009-
dc.identifier.rimsid54282-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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