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Broth microdilution method to detect extended-spectrum beta-lactamases and AmpC beta-lactamases in enterobacteriaceae isolates by use of clavulanic acid and boronic acid as inhibitors

Authors
 Seok Hoon Jeong  ;  Wonkeun Song  ;  Jae-Seok Kim  ;  Han-Sung Kim  ;  Kyu Man Lee 
Citation
 JOURNAL OF CLINICAL MICROBIOLOGY, Vol.47(11) : 3409-3412, 2009 
Journal Title
 JOURNAL OF CLINICAL MICROBIOLOGY 
ISSN
 0095-1137 
Issue Date
2009
MeSH
Anti-Bacterial Agents/pharmacology ; Boronic Acids/pharmacology* ; Clavulanic Acid/pharmacology* ; Culture Media/chemistry ; Enterobacteriaceae/drug effects* ; Enterobacteriaceae/enzymology* ; Enterobacteriaceae/isolation & purification ; Enterobacteriaceae Infections/microbiology ; Enzyme Inhibitors/pharmacology* ; Humans ; Microbial Sensitivity Tests/methods* ; Sensitivity and Specificity ; beta-Lactamase Inhibitors ; beta-Lactamases/biosynthesis*
Abstract
This study was designed to evaluate the performance of the broth microdilution (BMD) method to detect production of extended-spectrum beta-lactamases (ESBLs) and AmpC beta-lactamases in Enterobacteriaceae by using clavulanic acid (CA) and boronic acid (BA) as ESBL and AmpC beta-lactamase inhibitors, respectively. A total of 100 clinical isolates of Enterobacteriaceae were analyzed. Mueller-Hinton broth containing serial twofold dilutions of cefotaxime (CTX), ceftazidime (CAZ), aztreonam (ATM), or cefepime (FEP) with or without either or both CA and BA was prepared. An eightfold or greater decrease in the MIC of CTX, CAZ, ATM, or FEP in the presence of CA and BA was considered a positive result for ESBL and plasmid-mediated AmpC beta-lactamase (PABL), respectively. In tests with CA, expanded-spectrum beta-lactams containing BA (CTX-BA, CAZ-BA, ATM-BA, and FEP-BA) showed higher positive rates in detecting ESBL producers than those without BA. The combination of CTX- and CAZ-based BMD tests with CA and BA showed sensitivity and specificity of 100% for the detection of ESBLs and PABLs. The BMD testing could be applicable for routine use in commercially available semiautomated systems for the detection of ESBLs and PABLs in Enterobacteriaceae.
Files in This Item:
T200903687.pdf Download
DOI
10.1128/JCM.01141-09
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/105292
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