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알레르기 환자에서 TLR9 ligand인 CpG-ODN 자극에 의한 IFN-α 분비와 TLR9 발현

DC FieldValueLanguage
dc.contributor.author김규언-
dc.date.accessioned2015-04-24T17:17:01Z-
dc.date.available2015-04-24T17:17:01Z-
dc.date.issued2009-
dc.identifier.issn1738-1061-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/105104-
dc.description.abstractPURPOSE: The aim of this study is to explore the effect of the Toll-like receptor 9 (TLR9) expressed in plasmacytoid dendritic cells (pDCs) that respond to antigen to Th2 immune deviation in allergic patients. METHODS: Subjects consisted of 19 allergic patients and 17 healthy volunteers. Skin prick tests and nasal provocation tests were performed for the two groups. Peripheral blood mononuclear cells (PBMCs) were collected from subjects and analyzed for the Lineage Cocktail (CD3, CD14, CD16, CD19, CD20, CD56) (-), HLA-DR (+), and CD123 (+) using flow cytometry. In addition, we analyzed TLR9 mRNA by reverse transcriptase-polymerase chain reaction. The level of interferon-alpha (IFN-alpha) of the PBMCs following stimulation with the TLR9 ligand CpG-ODN 2216 was also evaluated. RESULTS: Analyses of CD123 (+) revealed a nearly similar distribution for the classical pDC markers in the allergic group (0.1%+/-0.04%) and in the controls (0.25%+/-0.23%). The mRNA levels of TLR9 on PBMCs were not different between the allergic group and the controls (1.29+/-0.41 vs. 1.25+/-0.23, respectively). Additionally, the level of IFN-alpha in PBMCs exposed to stimuli of the TLR9 ligand CpG-ODN 2216 was not significantly different between the two groups (911+/-829 vs. 1,095+/-888 pg/mL, respectively). CONCLUSIONS: We found no evidence that TLR9-dependent immune responses in human pDCs are associated with allergic status-
dc.description.statementOfResponsibilityopen-
dc.format.extent1015~1020-
dc.relation.isPartOfKorean Journal of Pediatrics-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.title알레르기 환자에서 TLR9 ligand인 CpG-ODN 자극에 의한 IFN-α 분비와 TLR9 발현-
dc.title.alternativeToll-like receptor 9 expression and interferon-alpha secretion upon CpG-ODN stimulation in allergic subjects-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pediatrics (소아과학)-
dc.contributor.googleauthor한만용-
dc.contributor.googleauthor지혜미-
dc.contributor.googleauthor김형윤-
dc.contributor.googleauthor이초애-
dc.contributor.googleauthor조효진-
dc.contributor.googleauthor황성규-
dc.contributor.googleauthor김규언-
dc.identifier.doi10.3345/kjp.2009.52.9.1015-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00327-
dc.relation.journalcodeJ02100-
dc.subject.keywordAllergy-
dc.subject.keywordToll-like receptor 9-
dc.subject.keywordPlasmacytoid dendritic cell-
dc.subject.keywordInterferon-alpha-
dc.contributor.alternativeNameKim, Kyu Earn-
dc.contributor.affiliatedAuthorKim, Kyu Earn-
dc.citation.volume52-
dc.citation.number9-
dc.citation.startPage1015-
dc.citation.endPage1020-
dc.identifier.bibliographicCitationKorean Journal of Pediatrics, Vol.52(9) : 1015-1020, 2009-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers

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