Cited 41 times in
Sustained high-level polyclonal hematopoietic marking and transgene expression 4 years after autologous transplantation of rhesus macaques with SIV lentiviral vector-transduced CD34+ cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김유진 | - |
dc.contributor.author | 홍범기 | - |
dc.date.accessioned | 2015-04-24T17:13:58Z | - |
dc.date.available | 2015-04-24T17:13:58Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0006-4971 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/105005 | - |
dc.description.abstract | We previously reported that lentiviral vectors derived from the simian immunodeficiency virus (SIV) were efficient at transducing rhesus hematopoietic repopulating cells. To evaluate the persistence of vector-containing and -expressing cells long term, and the safety implications of SIV lentiviral vector-mediated gene transfer, we followed 3 rhesus macaques for more than 4 years after transplantation with transduced CD34+ cells. All 3 animals demonstrated significant vector marking and expression of the GFP transgene in T cells, B cells, and granulocytes, with mean GFP+ levels of 6.7% (range, 3.3%-13.0%), 7.4% (4.2%-13.4%), and 5.6% (3.1%-10.5%), respectively. There was no vector silencing in hematopoietic cells over time. Vector insertion site analysis of granulocytes demonstrated sustained highly polyclonal reconstitution, with no evidence for progression to oligoclonality. A significant number of clones were found to contribute at both 1-year and 3- or 4-year time points. No vector integrations were detected in the MDS1/EVI1 region, in contrast to our previous findings with a gamma-retroviral vector. These data show that lentiviral vectors can mediate stable and efficient long-term expression in the progeny of transduced hematopoietic stem cells, with an integration profile that may be safer than that of standard Moloney murine leukemia virus (MLV)-derived retroviral vectors. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 5434~5443 | - |
dc.relation.isPartOf | BLOOD | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigens, CD34/metabolism* | - |
dc.subject.MESH | Biomarkers/blood* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Clone Cells | - |
dc.subject.MESH | Follow-Up Studies | - |
dc.subject.MESH | Gene Expression/physiology | - |
dc.subject.MESH | Genetic Therapy/methods | - |
dc.subject.MESH | Genetic Vectors/genetics | - |
dc.subject.MESH | Green Fluorescent Proteins/genetics | - |
dc.subject.MESH | Green Fluorescent Proteins/metabolism | - |
dc.subject.MESH | Hematopoietic Stem Cell Transplantation*/methods | - |
dc.subject.MESH | Hematopoietic Stem Cells/metabolism* | - |
dc.subject.MESH | Macaca mulatta | - |
dc.subject.MESH | Simian Immunodeficiency Virus/genetics* | - |
dc.subject.MESH | Simian Immunodeficiency Virus/metabolism | - |
dc.subject.MESH | Time Factors | - |
dc.subject.MESH | Transduction, Genetic | - |
dc.subject.MESH | Transgenes*/genetics | - |
dc.subject.MESH | Transplantation, Autologous | - |
dc.subject.MESH | Treatment Outcome | - |
dc.title | Sustained high-level polyclonal hematopoietic marking and transgene expression 4 years after autologous transplantation of rhesus macaques with SIV lentiviral vector-transduced CD34+ cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Yoo-Jin Kim | - |
dc.contributor.googleauthor | Yoon-Sang Kim | - |
dc.contributor.googleauthor | Andre Larochelle | - |
dc.contributor.googleauthor | Gabriel Renaud | - |
dc.contributor.googleauthor | Tyra G. Wolfsberg | - |
dc.contributor.googleauthor | Rima Adler | - |
dc.contributor.googleauthor | Robert E. Donahue | - |
dc.contributor.googleauthor | Peiman Hematti | - |
dc.contributor.googleauthor | Bum-Kee Hong | - |
dc.contributor.googleauthor | Jean Roayaei | - |
dc.contributor.googleauthor | Keiko Akagi | - |
dc.contributor.googleauthor | Janice M. Riberdy | - |
dc.contributor.googleauthor | Arthur W. Nienhuis | - |
dc.contributor.googleauthor | Cynthia E. Dunbar | - |
dc.contributor.googleauthor | Derek A. Persons | - |
dc.identifier.doi | 10.1182/blood-2008-10-185199 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04394 | - |
dc.contributor.localId | A00787 | - |
dc.relation.journalcode | J00341 | - |
dc.identifier.eissn | 1528-0020 | - |
dc.identifier.pmid | 19339698 | - |
dc.contributor.alternativeName | Kim, Yoo Jin | - |
dc.contributor.alternativeName | Hong, Bum Kee | - |
dc.contributor.affiliatedAuthor | Hong, Bum Kee | - |
dc.contributor.affiliatedAuthor | Kim, Yoo Jin | - |
dc.citation.volume | 113 | - |
dc.citation.number | 22 | - |
dc.citation.startPage | 5434 | - |
dc.citation.endPage | 5443 | - |
dc.identifier.bibliographicCitation | BLOOD, Vol.113(22) : 5434-5443, 2009 | - |
dc.identifier.rimsid | 55143 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.