Cited 69 times in
A distal cis-regulatory element, CNS-9, controls NFAT1 and IRF4-mediated IL-10 gene activation in T helper cells
DC Field | Value | Language |
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dc.contributor.author | 황기철 | - |
dc.date.accessioned | 2015-04-24T17:13:07Z | - |
dc.date.available | 2015-04-24T17:13:07Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0161-5890 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/104978 | - |
dc.description.abstract | IL-10 is a multifunctional cytokine that plays a critical role in maintaining the balance between immunity and tolerance. Previously, we identified proximal regulatory elements and alterations of chromatin structure in the IL-10 gene loci of Th1 and Th2 cells. We have now characterized a crucial cis-regulatory element, CNS-9, located 9kb upstream of the transcription start site in IL-10 gene loci. The CNS-9 region is highly conserved in vertebrate genomes, and contains clustered NFAT and IRF binding motifs. In vitro binding of NFAT1 and IRF4 to the CNS-9 region was observed by EMSA. Furthermore, Th2-preferential in vivo binding of NFAT1 and IRF4 to the CNS-9 region was observed by ChIP. Cyclosporine A treatment on wild type Th2 cells or Th2 cells derived from NFAT1 knockout (NFAT1(-/-)) mice showed significantly reduced trans-activity of CNS-9. The Th2 subset-specific enhancer activity of CNS-9 was upregulated synergistically by NFAT1 and its partner IRF4. Mutations in the binding sites for NFAT1 and IRF4 abrogated its enhancer activity of CNS-9. Collectively, our results establish crucial roles for enhancer element CNS-9, and NFAT1 and IRF4 that bind to it, for IL-10 expression in differential T helper subsets. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 613~621 | - |
dc.relation.isPartOf | MOLECULAR IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cyclosporine/pharmacology | - |
dc.subject.MESH | Enhancer Elements, Genetic/genetics* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interferon Regulatory Factors/genetics* | - |
dc.subject.MESH | Interferon Regulatory Factors/metabolism | - |
dc.subject.MESH | Interleukin-10/genetics* | - |
dc.subject.MESH | Interleukin-10/metabolism | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Mice, Knockout | - |
dc.subject.MESH | NFATC Transcription Factors/genetics* | - |
dc.subject.MESH | NFATC Transcription Factors/metabolism | - |
dc.subject.MESH | Signal Transduction/genetics | - |
dc.subject.MESH | Th1 Cells/metabolism | - |
dc.subject.MESH | Th2 Cells/drug effects | - |
dc.subject.MESH | Th2 Cells/immunology* | - |
dc.subject.MESH | Th2 Cells/metabolism | - |
dc.subject.MESH | Transcriptional Activation* | - |
dc.title | A distal cis-regulatory element, CNS-9, controls NFAT1 and IRF4-mediated IL-10 gene activation in T helper cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Life Science (의생명과학부) | - |
dc.contributor.googleauthor | Choong-Gu Lee | - |
dc.contributor.googleauthor | Kyu-Ho Kang | - |
dc.contributor.googleauthor | Jae-Seon So | - |
dc.contributor.googleauthor | Ho-Keun Kwon | - |
dc.contributor.googleauthor | Jun-Seock Son | - |
dc.contributor.googleauthor | Min-Kyung Song | - |
dc.contributor.googleauthor | Anupama Sahoo | - |
dc.contributor.googleauthor | Hwa-Joong Yi | - |
dc.contributor.googleauthor | Ki-Chul Hwang | - |
dc.contributor.googleauthor | Toshifumi Matsuyama | - |
dc.contributor.googleauthor | Katsuyuki Yui | - |
dc.contributor.googleauthor | Sin-Hyeog Im | - |
dc.identifier.doi | 10.1016/j.molimm.2008.07.037 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04456 | - |
dc.relation.journalcode | J02259 | - |
dc.identifier.eissn | 1872-9142 | - |
dc.identifier.pmid | 18962896 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0161589008003313 | - |
dc.subject.keyword | Th1/Th2 Cells | - |
dc.subject.keyword | Cytokines | - |
dc.subject.keyword | Transcription factors | - |
dc.subject.keyword | Gene regulation | - |
dc.subject.keyword | Molecular immunology | - |
dc.contributor.alternativeName | Hwang, Ki Chul | - |
dc.contributor.affiliatedAuthor | Hwang, Ki Chul | - |
dc.citation.volume | 46 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 613 | - |
dc.citation.endPage | 621 | - |
dc.identifier.bibliographicCitation | MOLECULAR IMMUNOLOGY, Vol.46(4) : 613-621, 2009 | - |
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