2 416

Cited 59 times in

A distal cis-regulatory element, CNS-9, controls NFAT1 and IRF4-mediated IL-10 gene activation in T helper cells

DC FieldValueLanguage
dc.contributor.author황기철-
dc.date.accessioned2015-04-24T17:13:07Z-
dc.date.available2015-04-24T17:13:07Z-
dc.date.issued2009-
dc.identifier.issn0161-5890-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/104978-
dc.description.abstractIL-10 is a multifunctional cytokine that plays a critical role in maintaining the balance between immunity and tolerance. Previously, we identified proximal regulatory elements and alterations of chromatin structure in the IL-10 gene loci of Th1 and Th2 cells. We have now characterized a crucial cis-regulatory element, CNS-9, located 9kb upstream of the transcription start site in IL-10 gene loci. The CNS-9 region is highly conserved in vertebrate genomes, and contains clustered NFAT and IRF binding motifs. In vitro binding of NFAT1 and IRF4 to the CNS-9 region was observed by EMSA. Furthermore, Th2-preferential in vivo binding of NFAT1 and IRF4 to the CNS-9 region was observed by ChIP. Cyclosporine A treatment on wild type Th2 cells or Th2 cells derived from NFAT1 knockout (NFAT1(-/-)) mice showed significantly reduced trans-activity of CNS-9. The Th2 subset-specific enhancer activity of CNS-9 was upregulated synergistically by NFAT1 and its partner IRF4. Mutations in the binding sites for NFAT1 and IRF4 abrogated its enhancer activity of CNS-9. Collectively, our results establish crucial roles for enhancer element CNS-9, and NFAT1 and IRF4 that bind to it, for IL-10 expression in differential T helper subsets.-
dc.description.statementOfResponsibilityopen-
dc.format.extent613~621-
dc.relation.isPartOfMOLECULAR IMMUNOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCyclosporine/pharmacology-
dc.subject.MESHEnhancer Elements, Genetic/genetics*-
dc.subject.MESHHumans-
dc.subject.MESHInterferon Regulatory Factors/genetics*-
dc.subject.MESHInterferon Regulatory Factors/metabolism-
dc.subject.MESHInterleukin-10/genetics*-
dc.subject.MESHInterleukin-10/metabolism-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHMice, Knockout-
dc.subject.MESHNFATC Transcription Factors/genetics*-
dc.subject.MESHNFATC Transcription Factors/metabolism-
dc.subject.MESHSignal Transduction/genetics-
dc.subject.MESHTh1 Cells/metabolism-
dc.subject.MESHTh2 Cells/drug effects-
dc.subject.MESHTh2 Cells/immunology*-
dc.subject.MESHTh2 Cells/metabolism-
dc.subject.MESHTranscriptional Activation*-
dc.titleA distal cis-regulatory element, CNS-9, controls NFAT1 and IRF4-mediated IL-10 gene activation in T helper cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Life Science (의생명과학부)-
dc.contributor.googleauthorChoong-Gu Lee-
dc.contributor.googleauthorKyu-Ho Kang-
dc.contributor.googleauthorJae-Seon So-
dc.contributor.googleauthorHo-Keun Kwon-
dc.contributor.googleauthorJun-Seock Son-
dc.contributor.googleauthorMin-Kyung Song-
dc.contributor.googleauthorAnupama Sahoo-
dc.contributor.googleauthorHwa-Joong Yi-
dc.contributor.googleauthorKi-Chul Hwang-
dc.contributor.googleauthorToshifumi Matsuyama-
dc.contributor.googleauthorKatsuyuki Yui-
dc.contributor.googleauthorSin-Hyeog Im-
dc.identifier.doi10.1016/j.molimm.2008.07.037-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA04456-
dc.relation.journalcodeJ02259-
dc.identifier.eissn1872-9142-
dc.identifier.pmid18962896-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0161589008003313-
dc.subject.keywordTh1/Th2 Cells-
dc.subject.keywordCytokines-
dc.subject.keywordTranscription factors-
dc.subject.keywordGene regulation-
dc.subject.keywordMolecular immunology-
dc.contributor.alternativeNameHwang, Ki Chul-
dc.contributor.affiliatedAuthorHwang, Ki Chul-
dc.citation.volume46-
dc.citation.number4-
dc.citation.startPage613-
dc.citation.endPage621-
dc.identifier.bibliographicCitationMOLECULAR IMMUNOLOGY, Vol.46(4) : 613-621, 2009-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.