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Qualitative and quantitative comparison of N-glycans between pig endothelial and islet cells by high-performance liquid chromatography and mass spectrometry-based strategy

DC FieldValueLanguage
dc.contributor.author김형일-
dc.date.accessioned2015-04-24T17:08:52Z-
dc.date.available2015-04-24T17:08:52Z-
dc.date.issued2009-
dc.identifier.issn1076-5174-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/104849-
dc.description.abstractN-glycan structures released from miniature pig endothelial and islet cells were determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), negative ion electrospray ionization (ESI) MS/MS and normal-phase high performance liquid chromatography (NP-HPLC) combined with exoglycosidase digestion. Totally, the identified structures were 181 N-glycans including 129 sialylated and 18 alpha-galactosylated glycans from pig endothelial cells and 80 N-glycans including 41 sialylated and one alpha-galactosylated glycans from pig islet cells. The quantity of the alpha-galactosylated glycans from pig islet cells was certainly neglectable compared to pig endothelial cells. A number of NeuGc-terminated N-glycans (80 from pig endothelial cells and 13 from pig islet cells) are newly detected by our mass spectrometric strategies. The detailed structural information will be a matter of great interest in organ or cell xenotransplantation using alpha 1,3-galactosyltransferase gene-knockout (GalT-KO) pig-
dc.description.statementOfResponsibilityopen-
dc.format.extent1087~1104-
dc.relation.isPartOfJOURNAL OF MASS SPECTROMETRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCell Line-
dc.subject.MESHChromatography, High Pressure Liquid/methods*-
dc.subject.MESHEndothelial Cells/chemistry*-
dc.subject.MESHEndothelial Cells/metabolism-
dc.subject.MESHGlycomics/methods*-
dc.subject.MESHGlycoside Hydrolases/metabolism-
dc.subject.MESHIslets of Langerhans/chemistry*-
dc.subject.MESHIslets of Langerhans/metabolism-
dc.subject.MESHPolysaccharides/chemistry*-
dc.subject.MESHPolysaccharides/metabolism-
dc.subject.MESHSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods*-
dc.subject.MESHSwine-
dc.titleQualitative and quantitative comparison of N-glycans between pig endothelial and islet cells by high-performance liquid chromatography and mass spectrometry-based strategy-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Surgery (외과학)-
dc.contributor.googleauthorYun-Gon Kim-
dc.contributor.googleauthorGeun-Cheol Gil-
dc.contributor.googleauthorKyung-Soon Jang-
dc.contributor.googleauthorSukmook Lee-
dc.contributor.googleauthorHyoung-Il Kim-
dc.contributor.googleauthorJung-Sik Kim-
dc.contributor.googleauthorJunho Chung-
dc.contributor.googleauthorChung-Gyu Park-
dc.contributor.googleauthorDavid J. Harveyh-
dc.contributor.googleauthorByung-Gee Kim-
dc.identifier.doi10.1002/jms.1587-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01154-
dc.relation.journalcodeJ01570-
dc.identifier.eissn1096-9888-
dc.identifier.pmid19373860-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/jms.1587/abstract-
dc.subject.keywordxenotransplantation-
dc.subject.keywordmass spectrometry-
dc.subject.keywordα-galactose-
dc.subject.keywordN-glycolyl-neuraminic acid-
dc.subject.keywordpig endothelial cell-
dc.subject.keywordpig islet cells-
dc.contributor.alternativeNameKim, Hyoung Il-
dc.contributor.affiliatedAuthorKim, Hyoung Il-
dc.citation.volume44-
dc.citation.number7-
dc.citation.startPage1087-
dc.citation.endPage1104-
dc.identifier.bibliographicCitationJOURNAL OF MASS SPECTROMETRY, Vol.44(7) : 1087-1104, 2009-
dc.identifier.rimsid42519-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

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