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Qualitative and quantitative comparison of N-glycans between pig endothelial and islet cells by high-performance liquid chromatography and mass spectrometry-based strategy

Authors
 Yun-Gon Kim  ;  Geun-Cheol Gil  ;  Kyung-Soon Jang  ;  Sukmook Lee  ;  Hyoung-Il Kim  ;  Jung-Sik Kim  ;  Junho Chung  ;  Chung-Gyu Park  ;  David J. Harveyh  ;  Byung-Gee Kim 
Citation
 JOURNAL OF MASS SPECTROMETRY, Vol.44(7) : 1087-1104, 2009 
Journal Title
 JOURNAL OF MASS SPECTROMETRY 
ISSN
 1076-5174 
Issue Date
2009
MeSH
Animals ; Cell Line ; Chromatography, High Pressure Liquid/methods* ; Endothelial Cells/chemistry* ; Endothelial Cells/metabolism ; Glycomics/methods* ; Glycoside Hydrolases/metabolism ; Islets of Langerhans/chemistry* ; Islets of Langerhans/metabolism ; Polysaccharides/chemistry* ; Polysaccharides/metabolism ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods* ; Swine
Keywords
xenotransplantation ; mass spectrometry ; α-galactose ; N-glycolyl-neuraminic acid ; pig endothelial cell ; pig islet cells
Abstract
N-glycan structures released from miniature pig endothelial and islet cells were determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), negative ion electrospray ionization (ESI) MS/MS and normal-phase high performance liquid chromatography (NP-HPLC) combined with exoglycosidase digestion. Totally, the identified structures were 181 N-glycans including 129 sialylated and 18 alpha-galactosylated glycans from pig endothelial cells and 80 N-glycans including 41 sialylated and one alpha-galactosylated glycans from pig islet cells. The quantity of the alpha-galactosylated glycans from pig islet cells was certainly neglectable compared to pig endothelial cells. A number of NeuGc-terminated N-glycans (80 from pig endothelial cells and 13 from pig islet cells) are newly detected by our mass spectrometric strategies. The detailed structural information will be a matter of great interest in organ or cell xenotransplantation using alpha 1,3-galactosyltransferase gene-knockout (GalT-KO) pig
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jms.1587/abstract
DOI
10.1002/jms.1587
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyoung Il(김형일) ORCID logo https://orcid.org/0000-0002-6134-4523
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104849
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