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The effect of composite pig islet-human endothelial cell grafts on the instant blood-mediated inflammatory reaction.
DC Field | Value | Language |
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dc.contributor.author | 김형일 | - |
dc.date.accessioned | 2015-04-24T17:08:46Z | - |
dc.date.available | 2015-04-24T17:08:46Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0963-6897 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/104846 | - |
dc.description.abstract | Instant blood-mediated inflammatory reaction (IBMIR) causes rapid islet loss in portal vein islet transplantation. Endothelial cells are known to protect against complement-mediated lysis and activation of coagulation. We tested composite pig islet-human endothelial cell grafts as a strategy to overcome IBMIR. Porcine islets were cocultured with human endothelial cells in specially modified culture medium composed of M199 and M200 for 1-9 days. A positive control group, negative control group, and the endothelial cell-coated group were examined with an in vitro tubing loop assay using human blood. The endothelial cell-coated group was subdivided and analyzed by degree of surface coverage by endothelial cells (< or = 50% vs. > 50%) or coculture time (< 5 days vs. > or = 5 days). Platelet consumption and complement and coagulation activation were assessed by platelet count, C3a, and thrombin-antithrombin complex (TAT), respectively. After 60-min incubation in human blood, the endothelial cell-coated group showed platelet consumption inhibition and low C3a and TAT assay results compared to uncoated controls. When the endothelial cell-coated group was subdivided by degree of surface coverage, the < or = 50% coated group showed less platelet consumption and less activation of complement and coagulation compared with the positive control (uncoated) group. On analysis by coculture time, only the subgroup cocultured for < 5 days showed the same protective effect. Human endothelial cell-coated pig islets, especially the partially coated and short-term cocultured pig islet-human endothelial cell composites, reduced all components of IBMIR. If the optimal endothelial cell-islet coculture method could be identified, human endothelial cell coating of pig islets would offer new strategies to improve xenogenic islet transplantation outcomes | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 31~37 | - |
dc.relation.isPartOf | CELL TRANSPLANTATION | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Endothelial Cells/immunology | - |
dc.subject.MESH | Endothelial Cells/transplantation* | - |
dc.subject.MESH | Graft Survival/immunology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Inflammation/blood | - |
dc.subject.MESH | Inflammation/immunology* | - |
dc.subject.MESH | Islets of Langerhans/cytology* | - |
dc.subject.MESH | Islets of Langerhans/immunology | - |
dc.subject.MESH | Islets of Langerhans Transplantation/immunology* | - |
dc.subject.MESH | Swine | - |
dc.subject.MESH | Transplantation, Heterologous/immunology* | - |
dc.title | The effect of composite pig islet-human endothelial cell grafts on the instant blood-mediated inflammatory reaction. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | Hyoung-Il Kim | - |
dc.contributor.googleauthor | Jae Eun Yu | - |
dc.contributor.googleauthor | Song Yi Lee | - |
dc.contributor.googleauthor | A. Young Sul | - |
dc.contributor.googleauthor | Min Seok Jang | - |
dc.contributor.googleauthor | M. A. Rashid | - |
dc.contributor.googleauthor | Sang Gyu Park | - |
dc.contributor.googleauthor | Sang Jun Kim | - |
dc.contributor.googleauthor | Chung-Gyu Park | - |
dc.contributor.googleauthor | Jae Hyeon Kim | - |
dc.contributor.googleauthor | Kyong Soo Park | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01154 | - |
dc.relation.journalcode | J00492 | - |
dc.identifier.eissn | 1555-3892 | - |
dc.identifier.pmid | 19476207 | - |
dc.identifier.url | http://www.ingentaconnect.com/content/cog/ct/2009/00000018/00000001/art00004 | - |
dc.contributor.alternativeName | Kim, Hyoung Il | - |
dc.contributor.affiliatedAuthor | Kim, Hyoung Il | - |
dc.citation.volume | 18 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 31 | - |
dc.citation.endPage | 37 | - |
dc.identifier.bibliographicCitation | CELL TRANSPLANTATION, Vol.18(1) : 31-37, 2009 | - |
dc.identifier.rimsid | 42516 | - |
dc.type.rims | ART | - |
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