Cited 16 times in
Interferon-gamma suppresses Na+ -H+ exchanger in cultured human endolymphatic sac epithelial cells
DC Field | Value | Language |
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dc.contributor.author | 김성헌 | - |
dc.contributor.author | 손은진 | - |
dc.contributor.author | 최재영 | - |
dc.date.accessioned | 2015-04-24T17:06:09Z | - |
dc.date.available | 2015-04-24T17:06:09Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0730-2312 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/104764 | - |
dc.description.abstract | Adequate regulation of endolymphatic pH is essential for maintaining inner ear function. the Na(+)-H(+) exchanger (NHE) is a major determinant of intracellular pH (pH(i)), and facilitates Na(+) and fluid absorption in various epithelia. We determined the functional and molecular expression of NHEs in cultured human endolymphatic sac (ES) epithelial cells and examined the effect of IFN-gamma on NHE function. Serial cultures of human ES epithelial cells were generated from tissue samples. the molecular expression of NHE1, -2, and -3 isoforms was determined by real-time RT-PCR. the functional activity of NHE isoforms was measured microfluorometrically using a pH-sensitive fluorescent dye, 2',7'-bis(carbonylethyl)-5(6)-carboxyfluorescein (BCECF), and a NHE-inhibitor, 3-methylsulfonyl-4-piperidinobenzoyl guanidine methanesulfonate (HOE694). NHE1, -2, and -3 mRNAs were expressed in human ES epithelial cells. Functional activity of NHE1 and -2 was confirmed in the luminal membrane of ES epithelial cells by sequentially suppressing Na(+)-dependent pH(i) recovery from intracellular acidification using different concentrations of HOE694. Treatment with IFN-gamma (50 nM for 24 h) suppressed mRNA expression of NHE1 and -2. IFN-gamma also suppressed functional activity of both NHE1 and -2 in the luminal membrane of ES epithelial cells. This study shows that NHEs are expressed in cultured human ES epithelial cells and that treatment with IFN-gamma suppresses the expression and functional activity of NHE1 and -2. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Buffers | - |
dc.subject.MESH | Cation Transport Proteins/genetics | - |
dc.subject.MESH | Cation Transport Proteins/metabolism | - |
dc.subject.MESH | Cattle | - |
dc.subject.MESH | Cell Shape/drug effects | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Endolymphatic Sac/cytology* | - |
dc.subject.MESH | Epithelial Cells/cytology* | - |
dc.subject.MESH | Epithelial Cells/drug effects* | - |
dc.subject.MESH | Epithelial Cells/metabolism | - |
dc.subject.MESH | Epithelial Cells/ultrastructure | - |
dc.subject.MESH | Gene Expression Regulation/drug effects | - |
dc.subject.MESH | HEPES | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hydrogen-Ion Concentration/drug effects | - |
dc.subject.MESH | Interferon-gamma/pharmacology* | - |
dc.subject.MESH | Protein Isoforms/genetics | - |
dc.subject.MESH | Protein Isoforms/metabolism | - |
dc.subject.MESH | Sodium/pharmacology | - |
dc.subject.MESH | Sodium-Hydrogen Exchanger 1 | - |
dc.subject.MESH | Sodium-Hydrogen Exchanger 3 | - |
dc.subject.MESH | Sodium-Hydrogen Exchangers/genetics | - |
dc.subject.MESH | Sodium-Hydrogen Exchangers/metabolism* | - |
dc.subject.MESH | Solutions | - |
dc.title | Interferon-gamma suppresses Na+ -H+ exchanger in cultured human endolymphatic sac epithelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Otorhinolaryngology (이비인후과학) | - |
dc.contributor.googleauthor | Eun Jin Son | - |
dc.contributor.googleauthor | In Seok Moon | - |
dc.contributor.googleauthor | Sung Huhn Kim | - |
dc.contributor.googleauthor | Su Jin Kim | - |
dc.contributor.googleauthor | Jae Young Choi | - |
dc.identifier.doi | 10.1002/jcb.22201 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04173 | - |
dc.contributor.localId | A00589 | - |
dc.contributor.localId | A01989 | - |
dc.relation.journalcode | J01303 | - |
dc.identifier.eissn | 1097-4644 | - |
dc.identifier.pmid | 19479940 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/jcb.22201/abstract | - |
dc.subject.keyword | ENDOLYMPHATIC SAC | - |
dc.subject.keyword | ION CHANNEL | - |
dc.subject.keyword | INTERFERON-g | - |
dc.subject.keyword | Naþ–HþEXCHANGER | - |
dc.contributor.alternativeName | Kim, Sung Huhn | - |
dc.contributor.alternativeName | Son, Eun Jin | - |
dc.contributor.alternativeName | Choi, Jae Young | - |
dc.contributor.affiliatedAuthor | Choi, Jae Young | - |
dc.contributor.affiliatedAuthor | Kim, Sung Huhn | - |
dc.contributor.affiliatedAuthor | Son, Eun Jin | - |
dc.citation.volume | 107 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 965 | - |
dc.citation.endPage | 972 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELLULAR BIOCHEMISTRY, Vol.107(5) : 965-972, 2009 | - |
dc.identifier.rimsid | 40191 | - |
dc.type.rims | ART | - |
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