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Interferon-gamma suppresses Na+ -H+ exchanger in cultured human endolymphatic sac epithelial cells

DC FieldValueLanguage
dc.contributor.author김성헌-
dc.contributor.author손은진-
dc.contributor.author최재영-
dc.contributor.author문인석-
dc.date.accessioned2015-04-24T17:06:09Z-
dc.date.available2015-04-24T17:06:09Z-
dc.date.issued2009-
dc.identifier.issn0730-2312-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/104764-
dc.description.abstractAdequate regulation of endolymphatic pH is essential for maintaining inner ear function. the Na(+)-H(+) exchanger (NHE) is a major determinant of intracellular pH (pH(i)), and facilitates Na(+) and fluid absorption in various epithelia. We determined the functional and molecular expression of NHEs in cultured human endolymphatic sac (ES) epithelial cells and examined the effect of IFN-gamma on NHE function. Serial cultures of human ES epithelial cells were generated from tissue samples. the molecular expression of NHE1, -2, and -3 isoforms was determined by real-time RT-PCR. the functional activity of NHE isoforms was measured microfluorometrically using a pH-sensitive fluorescent dye, 2',7'-bis(carbonylethyl)-5(6)-carboxyfluorescein (BCECF), and a NHE-inhibitor, 3-methylsulfonyl-4-piperidinobenzoyl guanidine methanesulfonate (HOE694). NHE1, -2, and -3 mRNAs were expressed in human ES epithelial cells. Functional activity of NHE1 and -2 was confirmed in the luminal membrane of ES epithelial cells by sequentially suppressing Na(+)-dependent pH(i) recovery from intracellular acidification using different concentrations of HOE694. Treatment with IFN-gamma (50 nM for 24 h) suppressed mRNA expression of NHE1 and -2. IFN-gamma also suppressed functional activity of both NHE1 and -2 in the luminal membrane of ES epithelial cells. This study shows that NHEs are expressed in cultured human ES epithelial cells and that treatment with IFN-gamma suppresses the expression and functional activity of NHE1 and -2.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF CELLULAR BIOCHEMISTRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBuffers-
dc.subject.MESHCation Transport Proteins/genetics-
dc.subject.MESHCation Transport Proteins/metabolism-
dc.subject.MESHCattle-
dc.subject.MESHCell Shape/drug effects-
dc.subject.MESHCells, Cultured-
dc.subject.MESHEndolymphatic Sac/cytology*-
dc.subject.MESHEpithelial Cells/cytology*-
dc.subject.MESHEpithelial Cells/drug effects*-
dc.subject.MESHEpithelial Cells/metabolism-
dc.subject.MESHEpithelial Cells/ultrastructure-
dc.subject.MESHGene Expression Regulation/drug effects-
dc.subject.MESHHEPES-
dc.subject.MESHHumans-
dc.subject.MESHHydrogen-Ion Concentration/drug effects-
dc.subject.MESHInterferon-gamma/pharmacology*-
dc.subject.MESHProtein Isoforms/genetics-
dc.subject.MESHProtein Isoforms/metabolism-
dc.subject.MESHSodium/pharmacology-
dc.subject.MESHSodium-Hydrogen Exchanger 1-
dc.subject.MESHSodium-Hydrogen Exchanger 3-
dc.subject.MESHSodium-Hydrogen Exchangers/genetics-
dc.subject.MESHSodium-Hydrogen Exchangers/metabolism*-
dc.subject.MESHSolutions-
dc.titleInterferon-gamma suppresses Na+ -H+ exchanger in cultured human endolymphatic sac epithelial cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Otorhinolaryngology (이비인후과학)-
dc.contributor.googleauthorEun Jin Son-
dc.contributor.googleauthorIn Seok Moon-
dc.contributor.googleauthorSung Huhn Kim-
dc.contributor.googleauthorSu Jin Kim-
dc.contributor.googleauthorJae Young Choi-
dc.identifier.doi10.1002/jcb.22201-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA04173-
dc.contributor.localIdA00589-
dc.contributor.localIdA01989-
dc.relation.journalcodeJ01303-
dc.identifier.eissn1097-4644-
dc.identifier.pmid19479940-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/jcb.22201/abstract-
dc.subject.keywordENDOLYMPHATIC SAC-
dc.subject.keywordION CHANNEL-
dc.subject.keywordINTERFERON-g-
dc.subject.keywordNaþ–HþEXCHANGER-
dc.contributor.alternativeNameKim, Sung Huhn-
dc.contributor.alternativeNameSon, Eun Jin-
dc.contributor.alternativeNameChoi, Jae Young-
dc.contributor.affiliatedAuthorChoi, Jae Young-
dc.contributor.affiliatedAuthorKim, Sung Huhn-
dc.contributor.affiliatedAuthorSon, Eun Jin-
dc.citation.volume107-
dc.citation.number5-
dc.citation.startPage965-
dc.citation.endPage972-
dc.identifier.bibliographicCitationJOURNAL OF CELLULAR BIOCHEMISTRY, Vol.107(5) : 965-972, 2009-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers

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