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Interferon-gamma suppresses Na+ -H+ exchanger in cultured human endolymphatic sac epithelial cells

 Eun Jin Son  ;  In Seok Moon  ;  Sung Huhn Kim  ;  Su Jin Kim  ;  Jae Young Choi 
 Journal of Cellular Biochemistry, Vol.107(5) : 965-972, 2009 
Journal Title
 Journal of Cellular Biochemistry 
Issue Date
Adequate regulation of endolymphatic pH is essential for maintaining inner ear function. the Na(+)-H(+) exchanger (NHE) is a major determinant of intracellular pH (pH(i)), and facilitates Na(+) and fluid absorption in various epithelia. We determined the functional and molecular expression of NHEs in cultured human endolymphatic sac (ES) epithelial cells and examined the effect of IFN-gamma on NHE function. Serial cultures of human ES epithelial cells were generated from tissue samples. the molecular expression of NHE1, -2, and -3 isoforms was determined by real-time RT-PCR. the functional activity of NHE isoforms was measured microfluorometrically using a pH-sensitive fluorescent dye, 2',7'-bis(carbonylethyl)-5(6)-carboxyfluorescein (BCECF), and a NHE-inhibitor, 3-methylsulfonyl-4-piperidinobenzoyl guanidine methanesulfonate (HOE694). NHE1, -2, and -3 mRNAs were expressed in human ES epithelial cells. Functional activity of NHE1 and -2 was confirmed in the luminal membrane of ES epithelial cells by sequentially suppressing Na(+)-dependent pH(i) recovery from intracellular acidification using different concentrations of HOE694. Treatment with IFN-gamma (50 nM for 24 h) suppressed mRNA expression of NHE1 and -2. IFN-gamma also suppressed functional activity of both NHE1 and -2 in the luminal membrane of ES epithelial cells. This study shows that NHEs are expressed in cultured human ES epithelial cells and that treatment with IFN-gamma suppresses the expression and functional activity of NHE1 and -2.
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1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sung Huhn(김성헌)
Moon, In Seok(문인석) ORCID logo https://orcid.org/0000-0002-3951-5074
Son, Eun Jin(손은진)
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
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