Cited 104 times in
Pretreatment of sildenafil attenuates ischemia-reperfusion renal injury in rats
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 임범진 | - |
dc.date.accessioned | 2015-04-24T17:05:15Z | - |
dc.date.available | 2015-04-24T17:05:15Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1931-857X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/104735 | - |
dc.description.abstract | Sildenafil was the first selective inhibitor of phosphodiesterase-5 (PDE5) to be widely used for treating erectile dysfunction. Many recent studies have investigated the cardioprotective role of sildenafil in animal models. We evaluated the protective effects of sildenafil in experimental renal ischemia-reperfusion (IR) injury in two studies. In study 1, male Sprague-Dawley rats were divided into four groups: sham, sildenafil-treated sham, vehicle-treated IR, and sildenafil-treated IR groups. In study 2, we divided the rats into two groups: sildenafil-treated IR rats and PD98059 (ERK inhibitor)+sildenafil-treated IR rats. Functional parameters of the kidney were evaluated at the molecular and structural levels. Blood urea nitrogen (BUN) and serum creatinine levels were lower in sildenafil-treated IR rats than in vehicle-treated IR rats. The expression of inducible (iNOS) and endothelial nitric oxide synthase (eNOS) proteins in sildenafil-treated IR rats was significantly higher than in vehicle-treated IR rats. Pretreatment with sildenafil in IR rats increased ERK phosphorylation and reduced the renal Bax/Bcl-2 ratio, renal caspase-3 activity, and terminal dUTP nick end-labeling-positive apoptotic cells. In contrast, PD98059 treatment increased BUN and serum creatinine levels and attenuated the sildenafil-induced expression of pERK, iNOS, eNOS, and Bcl-2. PD98059 also increased caspase-3 activity but did not decrease the sildenafil-induced accumulation of cGMP. In conclusion, this study suggests that sildenafil has antiapoptotic effects in experimental IR renal injury via ERK phosphorylation, induction of iNOS and eNOS production, and a decrease in the Bax/Bcl-2 ratio | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | F362-70 | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis/drug effects | - |
dc.subject.MESH | Blood Urea Nitrogen | - |
dc.subject.MESH | Caspase 3/metabolism | - |
dc.subject.MESH | Creatinine/blood | - |
dc.subject.MESH | Cyclic GMP/metabolism | - |
dc.subject.MESH | Cyclic Nucleotide Phosphodiesterases, Type 5/metabolism | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Drug Administration Schedule | - |
dc.subject.MESH | Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors | - |
dc.subject.MESH | Extracellular Signal-Regulated MAP Kinases/metabolism | - |
dc.subject.MESH | Flavonoids/administration & dosage | - |
dc.subject.MESH | Kidney Diseases/enzymology | - |
dc.subject.MESH | Kidney Diseases/pathology | - |
dc.subject.MESH | Kidney Diseases/prevention & control* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Nitric Oxide Synthase Type II/metabolism | - |
dc.subject.MESH | Nitric Oxide Synthase Type III/metabolism | - |
dc.subject.MESH | Phosphodiesterase 5 Inhibitors* | - |
dc.subject.MESH | Phosphodiesterase Inhibitors/administration & dosage* | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Piperazines/administration & dosage* | - |
dc.subject.MESH | Protein Kinase Inhibitors/administration & dosage | - |
dc.subject.MESH | Proto-Oncogene Proteins c-bcl-2/metabolism | - |
dc.subject.MESH | Purines/administration & dosage | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | ReperfusionInjury/enzymology | - |
dc.subject.MESH | ReperfusionInjury/pathology | - |
dc.subject.MESH | ReperfusionInjury/prevention & control* | - |
dc.subject.MESH | Sildenafil Citrate | - |
dc.subject.MESH | Sulfones/administration & dosage* | - |
dc.subject.MESH | Time Factors | - |
dc.subject.MESH | bcl-2-Associated X Protein/metabolism | - |
dc.title | Pretreatment of sildenafil attenuates ischemia-reperfusion renal injury in rats | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pathology (병리학) | - |
dc.contributor.googleauthor | Dae Eun Choi | - |
dc.contributor.googleauthor | Jin Young Jeong | - |
dc.contributor.googleauthor | Beom Jin Lim | - |
dc.contributor.googleauthor | Sarah Chung | - |
dc.contributor.googleauthor | Yoon Kyung Chang | - |
dc.contributor.googleauthor | Sang Ju Lee | - |
dc.contributor.googleauthor | Ki Ryang Na | - |
dc.contributor.googleauthor | Seok Young Kim | - |
dc.contributor.googleauthor | Young Tai Shin | - |
dc.contributor.googleauthor | Kang Wook Lee | - |
dc.identifier.doi | 10.1152/ajprenal.90609.2008 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03363 | - |
dc.relation.journalcode | J00108 | - |
dc.identifier.eissn | 1522-1466 | - |
dc.identifier.pmid | 19474186 | - |
dc.subject.keyword | apoptosis | - |
dc.subject.keyword | ERK | - |
dc.contributor.alternativeName | Lim, Beom Jin | - |
dc.contributor.affiliatedAuthor | Lim, Beom Jin | - |
dc.citation.volume | 297 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 362 | - |
dc.citation.endPage | 70 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, Vol.297(2) : 362-70, 2009 | - |
dc.identifier.rimsid | 40166 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.