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Dose-related cytoprotective effect of alpha-lipoic acid on hydrogen peroxide-induced oxidative stress to pancreatic beta cells

Authors
 BYUNG WAN LEE  ;  SOO JIN KWON  ;  HEE YOUNG CHAE  ;  JUN GOO KANG  ;  CHUL SIK KIM  ;  SEONG JIN LEE  ;  HYUNG JOON YOO  ;  JAE HYEON KIM  ;  KYONG SOO PARK  ;  SUNG-HEE IHM 
Citation
 FREE RADICAL RESEARCH, Vol.43(1) : 68-77, 2009 
Journal Title
 FREE RADICAL RESEARCH 
ISSN
 1071-5762 
Issue Date
2009
MeSH
Animals ; Antioxidants/pharmacology ; Cell Survival/drug effects ; Cells, Cultured ; Cytoprotection/drug effects* ; Dose-Response Relationship, Drug ; Drug Evaluation, Preclinical ; Glucose/pharmacology ; Hydrogen Peroxide/metabolism ; Hydrogen Peroxide/pharmacology* ; Insulin/secretion ; Insulin-Secreting Cells/drug effects* ; Insulin-Secreting Cells/metabolism ; Insulin-Secreting Cells/secretion ; JNK Mitogen-Activated Protein Kinases/metabolism ; Male ; Membrane Potential, Mitochondrial/drug effects ; Oxidative Stress/drug effects* ; Rats ; Rats, Sprague-Dawley ; Thioctic Acid/pharmacology*
Keywords
Akt phosphorylation ; antioxidant ; apoptosis ; hydrogen peroxide ; α-lipoic acid ; pancreatic beta-cell
Abstract
alpha-Lipoic acid (alpha-LA), an antioxidant used for diabetic polyneuropathy, was reported to induce AMP-activated protein kinase activation and reductions in insulin secretion in pancreatic beta-cells at high concentrations (> or = 500 micromol/l). This study investigated whether alpha-LA has a protective role under oxidative stress in beta-cells and its effect is dose-related. In INS-1 cells treated with alpha-LA (150-1200 micromol/l) for 24 h, alpha-LA itself (> or = 300 micromol/l) induced apoptotic death dose-dependently. However, pre-treatment with 150 and 300 micromol/l alpha-LA reduced the hydrogen peroxide-induced apoptosis in INS-1 cells and isolated islets. alpha-LA alleviated hydrogen peroxide-induced reactive oxygen species production, mitochondrial membrane depolarization and c-JNK activation in beta-cells. alpha-LA induced phosphoinositide 3-kinase-dependent Akt phosphorylation in INS-1 cells. While alpha-LA is harmful to beta-cells at high concentrations in vitro, it has potential cytoprotective effects on beta-cells under oxidative stress as in diabetes by its antioxidant properties and possibly by Akt phosphorylation at clinically relevant concentrations.
Full Text
http://informahealthcare.com/doi/abs/10.1080/10715760802590400
DOI
10.1080/10715760802590400
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chul Sik(김철식)
Lee, Byung Wan(이병완) ORCID logo https://orcid.org/0000-0002-9899-4992
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104607
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