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Evaluation of an immunochromatographic assay kit for rapid identification of Mycobacterium tuberculosis complex in clinical isolates.

Authors
 Mi Young Park  ;  Young Jin Kim  ;  Sang Hyun Hwang  ;  Hyoung Hoi Kim  ;  Eun Yup Lee  ;  Seok Hoon Jeong  ;  Chulhun L. Chang 
Citation
 JOURNAL OF CLINICAL MICROBIOLOGY, Vol.47(2) : 481-484, 2009 
Journal Title
 JOURNAL OF CLINICAL MICROBIOLOGY 
ISSN
 0095-1137 
Issue Date
2009
MeSH
Animals ; Antibodies, Monoclonal ; Bacteriological Techniques/methods* ; Chromatography, Affinity/methods* ; Humans ; Immunoassay ; Mice ; Mycobacterium tuberculosis/isolation & purification* ; Sensitivity and Specificity ; Tuberculosis/diagnosis* ; Tuberculosis/microbiology
Abstract
We evaluated a new immunochromatographic assay (ICA) using mouse monoclonal anti-MPT64 antibody for rapid discrimination between Mycobacterium tuberculosis and nontuberculous mycobacteria in clinical isolates. A study with mycobacteria and other organisms showed excellent sensitivity (approximately equal 99%) and specificity (100%) and an appropriate detection limit (10(5) CFU/ml) when tested with M. tuberculosis H37Rv. This ICA can simplify the identification of M. tuberculosis in clinical laboratories.
Files in This Item:
T200902837.pdf Download
DOI
10.1128/JCM.01253-08
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104432
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