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The survival of donor-derived cells in a successfully grafted corneal button 10 years after penetrating keratoplasty for lattice dystrophy

Authors
 Sun Woong Kim  ;  Hwan Young Lee  ;  Tae-im Kim  ;  Kyoung-Jin Shin  ;  Woo Ick Yang  ;  Eung Kweon Kim 
Citation
 OPHTHALMOLOGICA, Vol.223(6) : 396-400, 2009 
Journal Title
 OPHTHALMOLOGICA 
ISSN
 0030-3755 
Issue Date
2009
MeSH
Corneal Dystrophies, Hereditary/surgery* ; Corneal Transplantation* ; DNA/analysis ; Graft Survival/genetics* ; Humans ; In Situ Hybridization, Fluorescence/methods* ; Keratinocytes/cytology ; Keratinocytes/physiology ; Keratoplasty, Penetrating* ; Male ; Middle Aged ; Secondary Prevention
Keywords
Cornea transplantation ; Keratocytes ; Keratoplasty ; Lattice corneal dystrophy ; Recurrence of corneal dystrophy
Abstract
AIMS: To investigate the survival of donor-derived cells in a successfully grafted corneal button 10 years after penetrating keratoplasty for lattice dystrophy. METHODS: In 1996, a 48-year-old male with lattice corneal dystrophy underwent penetrating keratoplasty 3 times in the right eye within a 3-month interval. Nine years and 7 months later, the patient underwent a fourth penetrating keratoplasty. After surgery, the previous graft was analyzed to determine the origin of the cells. The epithelium and endothelium were removed, and then the button was dissected into 5 stromal blocks measuring 2 x 1.8 mm. Tissues underwent forensic genotyping using 16 markers (amelogenin for sex chromosomes and 15 autosomal short tandem repeats). Patient buccal tissue DNA was used as a control. RESULTS: The epithelium and buccal tissue contained identical DNA (i.e. recipient DNA). Similarly, the most peripheral stromal tissue contained only recipient DNA. In contrast, the most central stromal tissue only contained DNA of nonrecipient origin (presumably donor), while the stromal tissue between the periphery and center contained both recipient and nonrecipient DNA. CONCLUSIONS: The corneal stroma was infiltrated by surrounding recipient-derived keratocytes from the periphery. Therefore, more donor-derived cells had survived in the central stroma.
Full Text
http://www.karger.com/Article/FullText/228716
DOI
10.1159/000228716
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
Yonsei Authors
Kim, Sun Woong(김선웅)
Kim, Eung Kweon(김응권) ORCID logo https://orcid.org/0000-0002-1453-8042
Kim, Tae Im(김태임) ORCID logo https://orcid.org/0000-0001-6414-3842
Shin, Kyoung Jin(신경진) ORCID logo https://orcid.org/0000-0002-1059-9665
Yang, Woo Ick(양우익) ORCID logo https://orcid.org/0000-0002-6084-5019
Lee, Hwan Young(이환영)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104266
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