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각질형성세포에서 레티노인산(All-trans Retinoic Acid)에 의한 IL-8 발현의 기전
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김수찬 | - |
dc.contributor.author | 정예리 | - |
dc.date.accessioned | 2015-04-24T16:43:46Z | - |
dc.date.available | 2015-04-24T16:43:46Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 4944-4739 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/104062 | - |
dc.description.abstract | Background: Retinoic acid (RA) has been reported to induce the up-regulation of inflammatory cytokines such as IL-1, TNF-α and IL-8 in dermal fibroblasts and keratinocytes. There is no evidence to support a direct interaction between the RA-mediated transcriptional machinery and IL-8 gene transcription. Objective: The aim of this study is to clarify the mechanism of the up-regulation of IL-8 in keratinocytes by RA. Methods: The IL-1, IL-8, TNF-α and MCP-1 mRNA expressions in HaCaT cells stimulated by RA were measured by quantitative RT-PCR. The effects of a NF-κB inhibitor and IL-1 receptor antagonist (ra) on the IL-8 mRNA expression were measured by quantitative RT-PCR. Electrophoretic motility shift assay (EMSA) was conducted on the RA-stimulated HaCaT cells that were or were not treated with NF-κB inhibitor to measure the NF-κB binding activity in each group. The phospho-IκB activity in the HaCaT cells after stimulation with RA was also measured by Western blotting. Results: An up-regulation of the IL-8 gene expression by RA was demonstrated in the HaCaT cells. The inhibition assay revealed the involvement of the NF-κB binding site of the IL-8 gene in the RA-enhanced promoter activity. EMSA demonstrated that RA enhanced the formation of the DNA-NF-κB complex. There was no evidence to support IL-1 as an intermediate stimulus between the RA-mediated transcriptional machinery and IL-8 gene transcription. Western blot analysis revealed increased phospho-IκB activity in the HaCaT cells after stimulation with RA. Conclusion: Our result suggested that the IL-8 gene expression of HaCaT cells after RA stimulation is caused by the activation of IKK and the dissociation of IκB from NF-κB and the transcription of NF-κB in the nucleus | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 674~682 | - |
dc.relation.isPartOf | Korean Journal of Dermatology (대한피부과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 각질형성세포에서 레티노인산(All-trans Retinoic Acid)에 의한 IL-8 발현의 기전 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Dermatology (피부과학) | - |
dc.contributor.googleauthor | 정예리 | - |
dc.contributor.googleauthor | 강태원 | - |
dc.contributor.googleauthor | 오성민 | - |
dc.contributor.googleauthor | 정승용 | - |
dc.contributor.googleauthor | 김수찬 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03658 | - |
dc.contributor.localId | A00637 | - |
dc.relation.journalcode | J02000 | - |
dc.subject.keyword | HaCaT cells | - |
dc.subject.keyword | IL-8 | - |
dc.subject.keyword | NF-κB | - |
dc.subject.keyword | Retinoic acid | - |
dc.contributor.alternativeName | Kim, Soo Chan | - |
dc.contributor.alternativeName | Chung, Yae Lee | - |
dc.contributor.affiliatedAuthor | Chung, Yae Lee | - |
dc.contributor.affiliatedAuthor | Kim, Soo Chan | - |
dc.citation.volume | 47 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 674 | - |
dc.citation.endPage | 682 | - |
dc.identifier.bibliographicCitation | Korean Journal of Dermatology (대한피부과학회지), Vol.47(6) : 674-682, 2009 | - |
dc.identifier.rimsid | 46798 | - |
dc.type.rims | ART | - |
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