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The forkhead transcription factor Foxc2 stimulates osteoblast differentiation

Authors
 Se Hwa Kim  ;  Kyoung-Won Cho  ;  Han Seok Choi  ;  Su Jin Park  ;  Yumie Rhee  ;  Han-Sung Jung  ;  SAung-Kil Lim 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.386(3) : 532-536, 2009 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2009
MeSH
Animals ; Cell Differentiation* ; Cell Line ; Forkhead Transcription Factors/genetics ; Forkhead Transcription Factors/physiology* ; Mesenchymal Stromal Cells/cytology* ; Mesenchymal Stromal Cells/metabolism ; Mice ; Organ Culture Techniques ; Osteoblasts/cytology ; Osteoblasts/physiology* ; RNA, Small Interfering/genetics ; Skull/cytology ; Wnt Proteins/metabolism ; beta Catenin/metabolism
Keywords
Foxc2 ; Wnt ; β-catenin ; Osteoblast ; TCF/LEF ; Osteoblast differentiation
Abstract
The forkhead box C2 (Foxc2) protein is a member of the family of winged helix/forkhead transcription factors. Foxc2-deficient mice display defective formation of the aortic arches, multiple craniofacial bones, and vertebral columns. To investigate the role of Foxc2 in osteoblast differentiation, DNA containing Foxc2 was transfected into the developing cranial suture mesenchymal cells by electroporation. Compared to the controls, alkaline phosphatase (ALP) and bone sialoprotein were expressed strongly in suture mesenchymal cells in the Foxc2 overexpressed calvaria. After Foxc2-siRNA transfection, ALP staining was rarely observed in the suture mesenchyme and adjacent parietal bone of the calvaria. Meanwhile, overexpression of Foxc2 increased protein levels of beta-catenin and stimulated TCF/LEF transcriptional activity. The protein kinase A inhibitor H-89 suppressed Foxc2-mediated increases in TCF/LEF transcriptional activity (-40%, P<0.01). In conclusion, our results demonstrated that Foxc2 stimulated osteoblast differentiation of mesenchymal cells and preosteoblasts. Activation of canonical Wnt-beta-catenin signals might be involved in the Foxc2-mediated stimulation of osteoblast differentiation
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X09012236
DOI
10.1016/j.bbrc.2009.06.071
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Su Jin(박수진)
Rhee, Yumie(이유미) ORCID logo https://orcid.org/0000-0003-4227-5638
Lim, Sung Kil(임승길)
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Cho, Kyoung Won(조경원)
Choi, Han Seok(최한석)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104039
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