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Changes in expression of fibulin-5 and lysyl oxidase-like 1 associated with pelvic organ prolapse.

Authors
 Hyun Joo Jung  ;  Myung Jae Jeon  ;  Ga Won Yim  ;  Sei Kwang Kim  ;  Jong Rak Choi  ;  Sang Wook Bai 
Citation
 EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY, Vol.145(1) : 117-122, 2009 
Journal Title
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY
ISSN
 0301-2115 
Issue Date
2009
MeSH
Aged ; Amino Acid Oxidoreductases/metabolism* ; Case-Control Studies ; Connective Tissue/metabolism ; Elastic Tissue/metabolism ; Elastin/metabolism ; Extracellular Matrix Proteins/metabolism* ; Female ; Humans ; Korea ; Ligaments/metabolism* ; Middle Aged ; Prospective Studies ; RNA, Messenger/metabolism ; Uterine Prolapse/metabolism*
Keywords
Fibulin-5 ; Lysyl oxidase-like 1 ; Pelvic organ prolapseElastic fiber
Abstract
OBJECTIVES: Pelvic organ prolapse is associated with defects in connective tissue including elastic fibers. The purpose of this study was to investigate expression of fibulin-5 and lysyl oxidase-like 1, which play an essential role in synthesis and assembly of elastic fibers in the uterosacral ligament, in samples taken from women with advanced pelvic organ prolapse compared with controls.

STUDY DESIGN: Specimens were obtained prospectively during abdominal hysterectomy from 30 women with advanced pelvic organ prolapse and 30 controls matched to the study group for age and parity among postmenopausal women with benign gynecologic pathology. Expression levels of protein and mRNA of fibulin-5 and lysyl oxidase-like 1 in uterosacral ligaments were measured by Western blot analysis and real-time quantitative polymerase chain reactions. For statistical analyses, Student's t-test, chi-square test, Mann-Whitney U test, Fisher's exact test and Spearman's correlation were used.

RESULTS: Significant decrease in the expression of mRNA of fibulin-5 was found in patients with prolapse (P-value=0.042; 0.743+/-0.229 and 1.061+/-0.537). The expression of mRNA of lysyl oxidase-like 1 was increased in the patient group (P-value=0.017; 4.099+/-2.832 and 1.816+/-1.602). Similar results were shown in Western blot analysis. Differences in expression of fibulin-5 and lysyl oxidase-like 1 were noted according to stage of prolapse (P-value=0.037 and 0.009).

CONCLUSIONS: There was decreased expression of fibulin-5 and increased expression of lysyl oxidase-like 1 in uterosacral ligaments in patients with pelvic organ prolapse, which suggests the possibility of defects in elastin synthesis
Full Text
http://www.sciencedirect.com/science/article/pii/S0301211509002930
DOI
10.1016/j.ejogrb.2009.03.026
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sei Kwang(김세광)
Bai, Sang Wook(배상욱) ORCID logo https://orcid.org/0000-0001-7724-7552
Yim, Ga Won(임가원)
Jeon, Myung Jae(전명재)
Choi, Jong Rak(최종락) ORCID logo https://orcid.org/0000-0002-0608-2989
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103969
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