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The histone methyltransferase, NSD2, enhances androgen receptor-mediated transcription

Authors
 Hee-Bum Kang  ;  Youngsok Choi  ;  Jae Myun Lee  ;  Kyung-Chul Choi  ;  Han-Cheon Kim  ;  Jung-Yoon Yoo  ;  Yoo-Hyun Lee  ;  Ho-Geun Yoon 
Citation
 FEBS LETTERS, Vol.583(12) : 1880-1886, 2009 
Journal Title
 FEBS LETTERS 
ISSN
 0014-5793 
Issue Date
2009
MeSH
Active Transport, Cell Nucleus ; Base Sequence ; Cell Line, Tumor ; Chromatin Immunoprecipitation ; DNA Primers/genetics ; Enhancer Elements, Genetic ; Histone-Lysine N-Methyltransferase/chemistry ; Histone-Lysine N-Methyltransferase/genetics ; Histone-Lysine N-Methyltransferase/metabolism* ; Histones/metabolism ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; Humans ; Ligands ; Male ; Prostate-Specific Antigen/genetics ; Prostate-Specific Antigen/metabolism ; Prostatic Neoplasms/etiology ; Prostatic Neoplasms/genetics ; Prostatic Neoplasms/metabolism ; Protein Interaction Domains and Motifs ; Receptors, Androgen/chemistry ; Receptors, Androgen/genetics ; Receptors, Androgen/metabolism* ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Repressor Proteins/chemistry ; Repressor Proteins/genetics ; Repressor Proteins/metabolism* ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Transcriptional Activation
Keywords
Nuclear SET domain-containing 2 ; Androgen receptor ; Histone methyltransferase ; Prostate cancer ; Transcription
Abstract
In this study, we discovered that NSD2 specifically interacts with the DNA-binding domain of androgen receptor (AR) via its HMG domain, and the nuclear translocation of both NSD2 and AR is enhanced in the presence of ligand. Furthermore, we also demonstrated that the over expression of NSD2, but not of NSD2 (DeltaSET) HMT-activity defective mutant, enhanced the mRNA level of PSA in a dose-dependent manner. A chromatin immunoprecipitation assay showed that NSD2 protein is recruited to the enhancer region of the PSA gene by AR in an agonist-enhanced manner. Taken together, these results uncover a potential role for NSD2 in AR-mediated transcription, implicating NSD2 in prostate carcinogenesis.
Full Text
http://www.sciencedirect.com/science/article/pii/S001457930900413X
DOI
10.1016/j.febslet.2009.05.038
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kang, Hee Bum(강희범)
Kim, Han Cheon(김한천)
Yoo, Jung Yoon(유정윤) ORCID logo https://orcid.org/0000-0001-9366-3863
Yoon, Ho Geun(윤호근) ORCID logo https://orcid.org/0000-0003-2718-3372
Lee, Yoo Hyun(이유현)
Choi, Kyung Chul(최경철)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103910
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