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Induction of Apoptosis in Human Oral Epidermoid Carcinoma Cell by The Essential Oil of Artemisia Scoparia WALDST. et KITAMURA

Other Titles
 비쑥의 휘발성물질에 의한 KB 세포의 세포사멸 유도 
Authors
 Jeong-Dan Cha  ;  ShengJin, Li  ;  Myeong-Seop Lee  ;  In-Ho Cha 
Citation
 Korean Journal of Oral and Maxillofacial Pathology (대한구강악안면병리학회지), Vol.33(1) : 75-82, 2009 
Journal Title
 Korean Journal of Oral and Maxillofacial Pathology (대한구강악안면병리학회지) 
ISSN
 1225-1577 
Issue Date
2009
Keywords
Artemisia scoparia ; Essential oil ; Apoptosis ; Cell cycle ; Caspase-3 ; PARP
Abstract
Artemisia scoparia (A. scoparia), perennial herb is indigenous to Korea and has been traditionally used in liver damage. We investigated the effect of the essential oil obtained from A. scoparia on apoptosis of KB cells. Cytotoxicity and cellular DNA content were analyzed by MTT assay and flow cytometry, agarose gel electrophoresis, and Hoechst 33258 staining. The caspase-3 and poly (ADP-ribose) polymerase (PARP) proteins were estimated by Western blotting method. We found that the essential oil induced the apoptosis of the KB cells by concentrations of 0.4 to 0.2 mg/ml which was verified by DNA fragmentation, apoptotic bodies, and the sub-G0/G1 ratio. The essential oil also transient caspase-9 and caspase-3 activity and cleavage of PARP in KB cells for 24 h. The essential oil-induced apoptotic cell death was accompanied by up-regulation of Bax and down-regulation of Bcl-2. In conclusion, we demonstrated that the essential oil of A. scoparia induces apoptosis in KB cells.
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Appears in Collections:
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Myeong Seop(이명섭)
Li, Sheng Jin(이생금)
Cha, In Ho(차인호) ORCID logo https://orcid.org/0000-0001-8259-2190
Cha, Jeong Dan(차정단)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103780
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