Cited 20 times in
A new automated multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) using an AP720S analyzer
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김현숙 | - |
dc.date.accessioned | 2015-04-24T16:33:58Z | - |
dc.date.available | 2015-04-24T16:33:58Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0009-8981 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/103753 | - |
dc.description.abstract | BACKGROUND: In the diagnosis of atopic diseases, allergen detection is a crucial step. Multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) is a simple and noninvasive method for in vitro screening of allergen-specific IgE antibodies. METHODS: The Korean Inhalant Panel test on 20 patients and Food Panel test on 19 patients were performed using the conventional manual MAST-CLA kit and the new automated MAST-CLA method (automated AP720S system for the Optigen Assay; Hitachi Chemical Diagnostics, Inc., USA) simultaneously. The results were evaluated for positive reactivity and concordance. RESULTS: The results of inhalant panel gave a relatively higher class level result than the food panel. The 8 patients out of 20 (40%) of the inhalation panel, and 9 patients out of 18 (47.4%) of the food panel showed 100% concordance between the 2 systems. Eighteen patients (90%) of the Inhalation Panel and sixteen patients (84.2%) of the Food Panel showed more than 91% concordance. CONCLUSIONS: These results suggest that the MAST-CLA assay using the new, automated AP720S analyzer performs well, showing a high concordance rate with conventional MAST-CLA. Compared to manual MAST-CLA, the automated AP720S system has a shorter assay time and uses a smaller serum volume (500 microl) along with other conveniences. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 182~188 | - |
dc.relation.isPartOf | CLINICA CHIMICA ACTA | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adolescent | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Allergens/analysis* | - |
dc.subject.MESH | Child | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hypersensitivity/diagnosis* | - |
dc.subject.MESH | Hypersensitivity/immunology | - |
dc.subject.MESH | Immunoglobulin E/immunology* | - |
dc.subject.MESH | Luminescent Measurements/instrumentation* | - |
dc.subject.MESH | Luminescent Measurements/methods* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Young Adult | - |
dc.title | A new automated multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) using an AP720S analyzer | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Sungsil Lee | - |
dc.contributor.googleauthor | Hwan Sub Lim | - |
dc.contributor.googleauthor | Jungyong Park | - |
dc.contributor.googleauthor | Hyon Suk Kim | - |
dc.identifier.doi | 10.1016/j.cca.2009.01.014 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01117 | - |
dc.relation.journalcode | J00543 | - |
dc.identifier.eissn | 1873-3492 | - |
dc.identifier.pmid | 19263528 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0009898109000254 | - |
dc.subject.keyword | Multiple allergen simultaneous test (MAST) | - |
dc.subject.keyword | Chemiluminescent assay (CLA) | - |
dc.subject.keyword | Automated analyzer AP720S | - |
dc.subject.keyword | Allergy screening | - |
dc.subject.keyword | allergen-specific IgE | - |
dc.contributor.alternativeName | Kim, Hyon Suk | - |
dc.contributor.affiliatedAuthor | Kim, Hyon Suk | - |
dc.citation.volume | 402 | - |
dc.citation.number | 1-2 | - |
dc.citation.startPage | 182 | - |
dc.citation.endPage | 188 | - |
dc.identifier.bibliographicCitation | CLINICA CHIMICA ACTA, Vol.402(1-2) : 182-188, 2009 | - |
dc.identifier.rimsid | 36669 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.