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Comparative proteomic analysis of trophozoites versus cysts of Giardia lamblia.

Authors
 Juri Kim  ;  Sung-Su Bae  ;  Moon-Hee Sung  ;  Kyu-Ho Lee  ;  Soon-Jung Park 
Citation
 PARASITOLOGY RESEARCH, Vol.104(2) : 475-479, 2009 
Journal Title
 PARASITOLOGY RESEARCH 
ISSN
 0932-0113 
Issue Date
2009
MeSH
Animals ; Cysts/chemistry* ; Electrophoresis, Gel, Two-Dimensional ; Gene Expression Profiling ; Giardia lamblia/chemistry* ; Polymerase Chain Reaction/methods ; Proteome/analysis* ; Protozoan Proteins/analysis* ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Trophozoites/chemistry*
Abstract
The proteome of Giardia lamblia at its cyst stage was compared with that of trophozoites by using two-dimensional SDS-PAGE gel electrophoresis. Protein spots that increased in the extracts of cysts compared to trophozoites were identified by MALDI-TOF mass spectroscopy and categorized as cytoskeletal proteins, metabolic enzymes, a cell-cycle-specific kinase, stress resistance proteins, and a protein involved in translation. Expression patterns of five of the identified proteins were examined during encystation by real-time PCR. Expression of cwp1 (encoding cyst wall protein 1), a marker for encystation, was increased 11-fold. In contrast, tim (encoding triose-1-phosphate isomerase) was expressed constitutively during encystation, and its transcription level was therefore used as a mRNA loading control. Expression of three genes encoding beta-tubulin, vacuolar ATPase, and never-in-mitosis-A-related protein kinase did not vary significantly during encystation. Interestingly, genes encoding two heat shock proteins (Hsp70 and Hsp90) showed increased expression during encystation, suggesting that this differentiation process accompanies a cellular response to stress in G. lamblia.
Full Text
http://link.springer.com/article/10.1007%2Fs00436-008-1223-x
DOI
10.1007/s00436-008-1223-x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Ri(김주리) ORCID logo https://orcid.org/0000-0001-8270-7584
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103620
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