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Ultrastructural and temporal changes of the microvascular basement membrane and astrocyte interface following focal cerebral ischemia.

 Il Kwon  ;  Eun Hee Kim  ;  Gregory J. del Zoppo  ;  Ji Hoe Heo 
 JOURNAL OF NEUROSCIENCE RESEARCH, Vol.87(3) : 668-676, 2009 
Journal Title
Issue Date
Analysis of Variance ; Animals ; Astrocytes/physiology ; Astrocytes/ultrastructure* ; Basal Ganglia/blood supply ; Basal Ganglia/physiopathology ; Basal Ganglia/ultrastructure ; Basement Membrane/physiopathology ; Basement Membrane/ultrastructure* ; Infarction, Middle Cerebral Artery/pathology* ; Infarction, Middle Cerebral Artery/physiopathology ; Male ; Matrix Metalloproteinase 2/metabolism ; Matrix Metalloproteinase 9/metabolism ; Microscopy, Electron, Transmission ; Microvessels/physiopathology ; Microvessels/ultrastructure* ; Rats ; Rats, Sprague-Dawley ; Time Factors
cerebral ischemia ; astrocytes ; basement membrane ; electron microscopy
Microvascular integrity is lost during cerebral ischemia. Detachment of the microvascular basement membrane (BM) from the astrocyte, as well as degradation of the BM, is responsible for the loss of microvascular integrity. However, their ultrastructural and temporal changes during cerebral ischemia are not well known. Male Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (MCAO) for 1, 4, 8, 12, 16, 20, and 48 hr. By using transmission electron microscopy, the proportion of intact BM-astrocyte contacts and electron densities of the BM were measured from five randomly selected microvessels in the ischemic basal ganglia. Their temporal changes and associations with activities of the matrix metalloproteinases (MMPs) were investigated. The intact portion of the BM-astrocyte contacts was decreased significantly within 4 hr and was rarely observed at 48 hr after MCAO. Decreases in the electron density and degradation of the BM were significant 12 hr after MCAO. The intact BM-astrocyte contacts and the mean BM density showed a significant positive correlation (r = 0.784, P < 0.001). MMP-9 activity was correlated negatively with the intact BM-astrocyte contacts (r = -0.711, P < 0.001) and with the BM density (r = -0.538, P = 0.0016). The increase in MMP-9 coincided temporally with the loss of the BM-astrocyte contacts and a decrease in the BM density. Ultrastructural alterations occurring in the microvascular BM and its contacts with astrocyte endfeet were temporally associated in cerebral ischemia. Time courses of their alterations should be considered in the treatment targeted to the microvascular BM and its contact with astrocytes.
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1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Neurology (신경과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kwon, Il(권일) ORCID logo https://orcid.org/0000-0001-9449-5646
Kim, Eun Hee(김은희)
Heo, Ji Hoe(허지회) ORCID logo https://orcid.org/0000-0001-9898-3321
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