Cited 39 times in
The effect of Saccharomyces boulardii on human colon cells and inflammation in rats with trinitrobenzene sulfonic acid-induced colitis
DC Field | Value | Language |
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dc.contributor.author | 이상길 | - |
dc.date.accessioned | 2015-04-24T16:24:48Z | - |
dc.date.available | 2015-04-24T16:24:48Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0163-2116 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/103467 | - |
dc.description.abstract | Saccharomyces boulardii (S. boulardii) has beneficial effects in the treatment of intestinal inflammation; however, little is known about the mechanisms by which these effects occur. We investigated the effects of S. boulardii on the expression of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) and interleukin-8 (IL-8), using human HT-29 colonocytes and a rat model of trinitrobenzene sulfonic acid (TNBS)-induced colitis. The effect of S. boulardii on gene expression was assessed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and Northern blot and Western blot assays. Pharmacological inhibitors for various signaling pathways were used to determine the signaling pathways implicated in the S. boulardii regulation of PPAR-gamma and IL-8. We found that S. boulardii up-regulated and down-regulated PPAR-gamma and IL-8 expression at the transcription level, both in vitro and in vivo (P < 0.05, respectively). Saccharomyces boulardii blocked tumor necrosis factor-alpha (TNF-alpha) regulation of PPAR-gamma and IL-8 through disruption of TNF-alpha-mediated nuclear factor kappa B (NF-kappaB) activation. Furthermore, S. boulardii suppressed colitis and expression of pro-inflammatory cytokine genes in vivo (P < 0.05, respectively). Our study demonstrated that S. boulardii reduces colonic inflammation and regulates inflammatory gene expression. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 255~263 | - |
dc.relation.isPartOf | DIGESTIVE DISEASES AND SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Colitis/chemically induced | - |
dc.subject.MESH | Colitis/metabolism | - |
dc.subject.MESH | Colitis/therapy* | - |
dc.subject.MESH | Epithelial Cells/metabolism | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | HT29 Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-8/metabolism* | - |
dc.subject.MESH | Intestinal Mucosa/metabolism | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | NF-kappa B/metabolism | - |
dc.subject.MESH | PPAR gamma/metabolism* | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Saccharomyces/physiology* | - |
dc.subject.MESH | Trinitrobenzenesulfonic Acid/toxicity | - |
dc.subject.MESH | Tumor Necrosis Factor-alpha/metabolism | - |
dc.title | The effect of Saccharomyces boulardii on human colon cells and inflammation in rats with trinitrobenzene sulfonic acid-induced colitis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Sang Kil Lee | - |
dc.contributor.googleauthor | Youn Wha Kim | - |
dc.contributor.googleauthor | Sung-Gil Chi | - |
dc.contributor.googleauthor | Yeong-Shil Joo | - |
dc.contributor.googleauthor | Hyo Jong Kim | - |
dc.identifier.doi | 10.1007/s10620-008-0357-0 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02812 | - |
dc.relation.journalcode | J00737 | - |
dc.identifier.eissn | 1573-2568 | - |
dc.identifier.pmid | 18612822 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs10620-008-0357-0 | - |
dc.subject.keyword | Saccharomyces boulardii | - |
dc.subject.keyword | PPAR-γ | - |
dc.subject.keyword | IL-8 | - |
dc.subject.keyword | HT-29 | - |
dc.subject.keyword | TNBS-induced colitis | - |
dc.contributor.alternativeName | Lee, Sang Kil | - |
dc.contributor.affiliatedAuthor | Lee, Sang Kil | - |
dc.citation.volume | 54 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 255 | - |
dc.citation.endPage | 263 | - |
dc.identifier.bibliographicCitation | DIGESTIVE DISEASES AND SCIENCES, Vol.54(2) : 255-263, 2009 | - |
dc.identifier.rimsid | 36021 | - |
dc.type.rims | ART | - |
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