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Epidermal Langerhans cells rapidly capture and present antigens from C-type lectin-targeting antibodies deposited in the dermis

DC FieldValueLanguage
dc.contributor.author박채규-
dc.date.accessioned2015-04-23T17:51:49Z-
dc.date.available2015-04-23T17:51:49Z-
dc.date.issued2010-
dc.identifier.issn0022-202X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/103280-
dc.description.abstractAntigen-presenting cells can capture antigens that are deposited in the skin, including vaccines given subcutaneously. These include different dendritic cells (DCs) such as epidermal Langerhans cells (LCs), dermal DCs, and dermal langerin+ DCs. To evaluate access of dermal antigens to skin DCs, we used mAb to two C-type lectin endocytic receptors, DEC-205/CD205 and langerin/CD207. When applied to murine and human skin explant cultures, these mAbs were efficiently taken up by epidermal LCs. In addition, anti-DEC-205 targeted langerin+ CD103+ and langerin- CD103- mouse dermal DCs. Unexpectedly, intradermal injection of either mAb, but not isotype control, resulted in strong and rapid labeling of LCs in situ, implying that large molecules can diffuse through the basement membrane into the epidermis. Epidermal LCs targeted in vivo by ovalbumin-coupled anti-DEC-205 potently presented antigen to CD4+ and CD8+ T cells in vitro. However, to our surprise, LCs targeted through langerin were unable to trigger T-cell proliferation. Thus, epidermal LCs have a major function in uptake of lectin-binding antibodies under standard vaccination conditions-
dc.description.statementOfResponsibilityopen-
dc.format.extent755~762-
dc.relation.isPartOfJOURNAL OF INVESTIGATIVE DERMATOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAntibodies, Monoclonal/immunology-
dc.subject.MESHAntibodies, Monoclonal/pharmacology-
dc.subject.MESHAntigen Presentation/immunology*-
dc.subject.MESHAntigens, Surface/immunology*-
dc.subject.MESHCell Division/immunology-
dc.subject.MESHCell Movement/immunology-
dc.subject.MESHCells, Cultured-
dc.subject.MESHDermis/cytology-
dc.subject.MESHDermis/immunology*-
dc.subject.MESHEpidermal Cells-
dc.subject.MESHEpidermis/immunology*-
dc.subject.MESHHumans-
dc.subject.MESHInjections, Intradermal-
dc.subject.MESHLangerhans Cells/cytology-
dc.subject.MESHLangerhans Cells/immunology*-
dc.subject.MESHLectins, C-Type/immunology*-
dc.subject.MESHMannose-Binding Lectins/immunology*-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred BALB C-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHT-Lymphocytes/cytology-
dc.subject.MESHT-Lymphocytes/immunology-
dc.titleEpidermal Langerhans cells rapidly capture and present antigens from C-type lectin-targeting antibodies deposited in the dermis-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Life Science (의생명과학부)-
dc.contributor.googleauthorVincent Flacher-
dc.contributor.googleauthorChristoph H. Tripp-
dc.contributor.googleauthorPatrizia Stoitzner-
dc.contributor.googleauthorBernhard Haid-
dc.contributor.googleauthorSusanne Ebner-
dc.contributor.googleauthorFranz Koch-
dc.contributor.googleauthorChae Gyu Park-
dc.contributor.googleauthorRalph M. Steinman-
dc.contributor.googleauthorJuliana Idoyaga-
dc.contributor.googleauthorNikolaus Romani-
dc.identifier.doi10.1038/jid.2009.343-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01718-
dc.relation.journalcodeJ01469-
dc.identifier.eissn1523-1747-
dc.identifier.pmid19890348-
dc.contributor.alternativeNamePark, Chae Gyu-
dc.contributor.affiliatedAuthorPark, Chae Gyu-
dc.citation.volume130-
dc.citation.number3-
dc.citation.startPage755-
dc.citation.endPage762-
dc.identifier.bibliographicCitationJOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol.130(3) : 755-762, 2010-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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