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Epidermal Langerhans cells rapidly capture and present antigens from C-type lectin-targeting antibodies deposited in the dermis

Authors
 Vincent Flacher  ;  Christoph H. Tripp  ;  Patrizia Stoitzner  ;  Bernhard Haid  ;  Susanne Ebner  ;  Franz Koch  ;  Chae Gyu Park  ;  Ralph M. Steinman  ;  Juliana Idoyaga  ;  Nikolaus Romani 
Citation
 JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol.130(3) : 755-762, 2010 
Journal Title
 JOURNAL OF INVESTIGATIVE DERMATOLOGY 
ISSN
 0022-202X 
Issue Date
2010
MeSH
Animals ; Antibodies, Monoclonal/immunology ; Antibodies, Monoclonal/pharmacology ; Antigen Presentation/immunology* ; Antigens, Surface/immunology* ; Cell Division/immunology ; Cell Movement/immunology ; Cells, Cultured ; Dermis/cytology ; Dermis/immunology* ; Epidermal Cells ; Epidermis/immunology* ; Humans ; Injections, Intradermal ; Langerhans Cells/cytology ; Langerhans Cells/immunology* ; Lectins, C-Type/immunology* ; Mannose-Binding Lectins/immunology* ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; T-Lymphocytes/cytology ; T-Lymphocytes/immunology
Abstract
Antigen-presenting cells can capture antigens that are deposited in the skin, including vaccines given subcutaneously. These include different dendritic cells (DCs) such as epidermal Langerhans cells (LCs), dermal DCs, and dermal langerin+ DCs. To evaluate access of dermal antigens to skin DCs, we used mAb to two C-type lectin endocytic receptors, DEC-205/CD205 and langerin/CD207. When applied to murine and human skin explant cultures, these mAbs were efficiently taken up by epidermal LCs. In addition, anti-DEC-205 targeted langerin+ CD103+ and langerin- CD103- mouse dermal DCs. Unexpectedly, intradermal injection of either mAb, but not isotype control, resulted in strong and rapid labeling of LCs in situ, implying that large molecules can diffuse through the basement membrane into the epidermis. Epidermal LCs targeted in vivo by ovalbumin-coupled anti-DEC-205 potently presented antigen to CD4+ and CD8+ T cells in vitro. However, to our surprise, LCs targeted through langerin were unable to trigger T-cell proliferation. Thus, epidermal LCs have a major function in uptake of lectin-binding antibodies under standard vaccination conditions
Files in This Item:
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DOI
10.1038/jid.2009.343
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Park, Chae Gyu(박채규) ORCID logo https://orcid.org/0000-0003-1906-1308
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103280
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