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Control of adipogenesis by the SUMO-specific protease SENP2.

Authors
 Sung Soo Chung  ;  Byung Yong Ahn  ;  Min Kim  ;  Hye Hun Choi  ;  Ho Seon Park  ;  Shinae Kang  ;  Sang Gyu Park  ;  Young-Bum Kim  ;  Young Min Cho  ;  Hong Kyu Lee  ;  Chin Ha Chung  ;  Kyong Soo Park 
Citation
 MOLECULAR AND CELLULAR BIOLOGY, Vol.30(9) : 2135-2146, 2010 
Journal Title
 MOLECULAR AND CELLULAR BIOLOGY 
ISSN
 0270-7306 
Issue Date
2010
MeSH
3T3-L1 Cells ; Adipocytes/cytology ; Adipocytes/enzymology ; Adipogenesis* ; Animals ; CCAAT-Enhancer-Binding Protein-alpha/metabolism ; CCAAT-Enhancer-Binding Protein-beta/metabolism ; Cyclic AMP-Dependent Protein Kinases/metabolism ; Cysteine Endopeptidases ; Enzyme Activation ; Gene Expression Regulation ; Mice ; Multienzyme Complexes/genetics ; Multienzyme Complexes/metabolism* ; PPAR gamma/metabolism ; Peptide Hydrolases/metabolism* ; Protein Stability ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Small Interfering/metabolism ; Response Elements/genetics ; Small Ubiquitin-Related Modifier Proteins/metabolism* ; Substrate Specificity ; Ubiquitination
Abstract
Here, we demonstrate that SENP2, a desumoylating enzyme, plays a critical role in the control of adipogenesis. SENP2 expression was markedly increased upon the induction of adipocyte differentiation, and this increase was dependent on protein kinase A activation. Remarkably, knockdown of SENP2 led to a dramatic attenuation of adipogenesis with a marked decrease in PPARgamma and C/EBPalpha mRNA levels. Knockdown of SENP2 also caused a marked reduction in the level of C/EBPbeta protein but not in that of C/EBPbeta mRNA. Interestingly, sumoylation of C/EBPbeta dramatically increased its ubiquitination and destabilization, and this increase could be reversed by SENP2. In addition, overexpression of C/EBPbeta could overcome the inhibitory effect of SENP2 knockdown on adipogenesis. Furthermore, SENP2 was absolutely required for adipogenesis of preadipocytes implanted into mice. These results establish a critical role for SENP2 in the regulation of adipogenesis by desumoylation and stabilization of C/EBPbeta and in turn by promoting the expression of its downstream effectors, such as PPARgamma and C/EBPalpha
Files in This Item:
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DOI
10.1128/MCB.00852-09
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Shin Ae(강신애) ORCID logo https://orcid.org/0000-0002-9719-4774
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103260
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