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Nested PCR in lung tissue for diagnosis of pulmonary tuberculosis.

Authors
 J.S. Park  ;  Y.A. Kang  ;  S.Y. Kwon  ;  H.I. Yoon  ;  J-H. Chung  ;  C-T. Lee  ;  J.H. Lee 
Citation
 EUROPEAN RESPIRATORY JOURNAL, Vol.35(4) : 851-857, 2010 
Journal Title
EUROPEAN RESPIRATORY JOURNAL
ISSN
 0903-1936 
Issue Date
2010
MeSH
Adult ; Aged ; Aged, 80 and over ; Biopsy ; Female ; Humans ; Male ; Microbiological Techniques ; Middle Aged ; Mycobacterium tuberculosis/genetics* ; Mycobacterium tuberculosis/isolation & purification* ; Paraffin Embedding ; Polymerase Chain Reaction* ; Predictive Value of Tests ; Retrospective Studies ; Sensitivity and Specificity ; Tuberculosis, Pulmonary/diagnosis* ; Tuberculosis, Pulmonary/microbiology* ; Tuberculosis, Pulmonary/pathology ; Young Adult
Keywords
Diagnosis ; lung ; nested PCR ; pulmonary tuberculosis ; tissue
Abstract
Diagnosis of pulmonary tuberculosis (TB) is difficult in cases with an unusual presentation and often requires a lung biopsy. The goal of this study was to determine the clinical usefulness of nested PCR on lung tissue for the diagnosis of pulmonary TB. Clinical and laboratory data were reviewed from patients who underwent diagnostic lung biopsies, followed by nested TB PCR on formalin-fixed paraffin-embedded lung tissue specimens. The diagnostic yield and clinical impact of nested PCR were investigated. Of the 223 patients studied, 142 were diagnosed with TB. Microbiologically confirmed TB was identified in 71 patients. Compared to culture results, the sensitivity, specificity, positive predictive value and negative predictive value of nested PCR were 85%, 99%, 98% and 88%, respectively. Nested PCR was more sensitive than acid-fast bacilli smear of respiratory specimens and histopathological findings. The PCR results provided an early diagnosis and initiation of treatment for TB. However, negative PCR results did not lead to discontinuation of unnecessary TB treatment in patients on medication. In conclusion, nested PCR on lung tissue specimens is a useful diagnostic test for pulmonary TB in patients with an unusual presentation.
Files in This Item:
T201005869.pdf Download
DOI
10.1183/09031936.00067209
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Young Ae(강영애) ORCID logo https://orcid.org/0000-0002-7783-5271
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103236
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