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Therapeutic potential of bone-marrow-derived mesenchymal stem cells differentiated with growth-factor-free coculture method in liver-injured rats

DC FieldValueLanguage
dc.contributor.author김경식-
dc.contributor.author서활-
dc.contributor.author이혜선-
dc.contributor.author조현희-
dc.date.accessioned2015-04-23T17:33:30Z-
dc.date.available2015-04-23T17:33:30Z-
dc.date.issued2010-
dc.identifier.issn1937-3341-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/102697-
dc.description.abstractMesenchymal stem cell (MSC) differentiation by growth factors may be improper due to possibility of clinical risk. We have previously developed a growth-factor-free coculture method and observed rat MSCs differentiated into hepatic progenitor cells. This study was aimed to validate hepatic differentiation potential in vivo. MSCs from bone marrow of green fluorescent protein-transgenic Sprague-Dawley rats were cocultured with hepatocytes from normal Sprague-Dawley rats, sharing growth-factor-free media. After 14 days, cells were implanted into the spleen of carbon tetrachloride (CCl4)-injured rats and kept for 4 weeks. Fibrosis remarkably decreased in CCl4/cocultured MSC at weeks 1, 3, and 4. Immunohistochemistry revealed that albumin, alpha-fetoprotein, and cytokeratin 19 (CK19) expression was high in CCl4/cocultured MSC only at week 1. Reverse transcription-polymerase chain reaction and Western blot revealed that CCl4/cocultured MSC had reduced alpha-fetoprotein expression at week 4, whereas CK18 and CK19 exhibited stronger expression. Albumin in CCl4/cocultured MSC increased at week 4 only in protein level. We assume that cocultured MSCs had stayed at hepatic progenitor stage until week 3, and differentiated into hepatocytes or bile-ductal epithelial cells afterward. Hepatic progenitor cells from MSC differentiation in the growth-factor-free coculture system may contribute to the therapeutic effect for liver disease in vivo-
dc.description.statementOfResponsibilityopen-
dc.format.extent2649~2659-
dc.relation.isPartOfTISSUE ENGINEERING PART A-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBone Marrow Cells/cytology-
dc.subject.MESHBone Marrow Transplantation/methods*-
dc.subject.MESHCell Differentiation-
dc.subject.MESHCoculture Techniques-
dc.subject.MESHHepatocytes/cytology*-
dc.subject.MESHHepatocytes/transplantation*-
dc.subject.MESHIntercellular Signaling Peptides and Proteins-
dc.subject.MESHLiver Cirrhosis/pathology*-
dc.subject.MESHLiver Cirrhosis/surgery*-
dc.subject.MESHMale-
dc.subject.MESHMesenchymal Stem Cell Transplantation/methods*-
dc.subject.MESHMesenchymal Stromal Cells/cytology*-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHTreatment Outcome-
dc.titleTherapeutic potential of bone-marrow-derived mesenchymal stem cells differentiated with growth-factor-free coculture method in liver-injured rats-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Surgery (외과학)-
dc.contributor.googleauthorTian Zhu Li-
dc.contributor.googleauthorJae Hyung Kim-
dc.contributor.googleauthorHyun Hee Cho-
dc.contributor.googleauthorHye Sun Lee-
dc.contributor.googleauthorKyung Sik Kim-
dc.contributor.googleauthorSang Woo Lee-
dc.contributor.googleauthorHwal Suh-
dc.identifier.doi10.1089/ten.tea.2009.0814-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00299-
dc.contributor.localIdA01924-
dc.contributor.localIdA03934-
dc.contributor.localIdA03311-
dc.relation.journalcodeJ02735-
dc.identifier.eissn1937-335X-
dc.identifier.pmid20367252-
dc.identifier.urlhttp://online.liebertpub.com/doi/abs/10.1089/ten.TEA.2009.0814-
dc.contributor.alternativeNameKim, Kyung Sik-
dc.contributor.alternativeNameSuh, Hwal-
dc.contributor.alternativeNameLee, Hye Sun-
dc.contributor.alternativeNameCho, Hyun Hee-
dc.contributor.affiliatedAuthorKim, Kyung Sik-
dc.contributor.affiliatedAuthorSuh, Hwal-
dc.contributor.affiliatedAuthorCho, Hyun Hee-
dc.contributor.affiliatedAuthorLee, Hye Sun-
dc.citation.volume16-
dc.citation.number8-
dc.citation.startPage2649-
dc.citation.endPage2659-
dc.identifier.bibliographicCitationTISSUE ENGINEERING PART A, Vol.16(8) : 2649-2659, 2010-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers

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