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The oocyte-specific transcription factor, Nobox, regulates the expression of Pad6, a peptidylarginine deiminase in the oocyte

Authors
 Mikyung Choi  ;  Ok-Hee Lee  ;  Sanghyun Jeon  ;  Miseon Park  ;  Dong Ryul Lee  ;  Jeong-Jae Ko  ;  Tae Ki Yoon  ;  Aleksandar Rajkovic  ;  Youngsok Choi 
Citation
 FEBS LETTERS, Vol.584(16) : 3629-3634, 2010 
Journal Title
FEBS LETTERS
ISSN
 0014-5793 
Issue Date
2010
MeSH
Animals ; Animals, Newborn ; Base Sequence ; DNA Primers/genetics ; Female ; Gene Expression Regulation, Developmental ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism* ; Hydrolases/genetics* ; Hydrolases/metabolism* ; In Vitro Techniques ; Mice ; Mice, Knockout ; Oocytes/growth & development ; Oocytes/metabolism* ; Oogenesis/genetics ; Oogenesis/physiology ; Ovary/cytology ; Ovary/embryology ; Ovary/growth & development ; Ovary/metabolism ; Promoter Regions, Genetic ; Protein-Arginine Deiminases ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism ; Transcription Factors/deficiency ; Transcription Factors/genetics ; Transcription Factors/metabolism* ; Transcriptional Activation
Keywords
Nobox ; Folliculogenesis ; Pad6 ; Transcription
Abstract
Nobox is an oocyte-specific transcriptional regulator. Nobox deficiency disrupts early folliculogenesis and the expression of oocyte-specific genes in mice. In the present study, we found that peptidylarginine deiminase 6 (Pad6) was downregulated in Nobox-null ovaries. Pad6 is preferentially expressed in oocytes and its transcript is detectable at embryonic day 16.5. In addition, we identified one Nobox DNA-binding element (NBE) within the mouse Pad6 promoter. The NBE includes a core sequence TAATTA. Sequence-specific binding of Nobox to the TAATTA motif was confirmed. Nobox overexpression augmented transcriptional activity of a luciferase reporter driven by mouse Pad6. Our findings indicate that Nobox is a critical regulator that orchestrates oocyte-specific genes such as Pad6 during folliculogenesis.
Full Text
http://www.sciencedirect.com/science/article/pii/S0014579310005958
DOI
10.1016/j.febslet.2010.07.037
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
Yonsei Authors
Lee, Ok Hee(이옥희)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102378
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